Flashcards
HT 12 Flotation Bath Troubleshooting
Cards in this deck (12)
- 1Cross contaminationThe water surface is always skimmed between specimens to avoid contamination of one section with cells from another.
Care is taken not to brush hair or hands whilst floating-out sections (squames can contaminate sections). - 2debrissquames
- 3cross contamination /debristissue contamination
ex: thyroid tissue in cardiac section
can lead to misdiagnosis - 4folds
- 5wrinkle
- 6wavy sectionnot flat
- 7parched earthwater bath too hot
or not optimal processing
- 8air bubblesSmall air bubbles in the flotation bath. Any bubbles that are trapped under the section apparently disappear as the section dries. Although the bubbles may apparently disappear, the areas in the section above the bubble are often distorted and are likely to float off during staining.
- 9incomplete deparaffination
- 10retraction/nuclear bubblingattributed to presence of water in nuclei freshly cut and immediately dry (above 60C)
- 11retraction/shrinkage
- 12Adhesive artifactex excessive albumin
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- Flashcards on HT 12 Flotation Bath Troubleshooting, created by Vilmida Castro on 10/06/2019.
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- Vilmida Castro
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