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Flashcards

Microbiology-Bacteriology

Jaylene Brown English 91 cards

Cards in this deck (91)

  1. 1Gram + Cocci in clusters "bunches of grapes" Yellow/white, smooth, creamy colonies on SBA, can show hemolysis Catalase + Coagulase + Non-motile Non-sporeforming Facultative anaerobe Ferments mannitolStaphylococcus aureus
  2. 2Gram + cocci Smooth, creamy, White, nonhemolytic colonies on SBA Catalase + Coagulase - Oxidase - Bacitracin ResistantCoNS (Coagulase-negative Staphylococci) such as Staphylococcus epidermidis
  3. 3Gram + cocci Smooth, creamy, may have yellow pigment on SBA Catalase + Coagulase - Oxidase - Bacitracin & Novobicin ResistantStaphylococcus saprophyticus
  4. 4Gram + Cocci Catalase + Oxidase + Bacitracin Susceptible Coagulase -Micrococcus spp.
  5. 5Gram + cocci in chains Transparent, clear colonies w/ large zone of beta-hemolysis on SBA Facultative anaerobe Oxidase - Catalase - Hyalronic acid capsule Streptolysin O, DNase Bacitracin (A disc) susceptible SXT Resistant PYR hydrolysis + Bile esculin - 6.5% NaCl -Streptococcus pyogenes (Group A Strep)
  6. 6Gram + cocci Grayish white, creamy colonies w/ narrow zone of beta-hemolysis on SBA Facultative anaerobe Oxidase - Catalase - Bacitracin Resistant CAMP + (arrow-shaped) Bile esculin - 6.5% NaCl - Hippurate hydrolysis +Streptococcus agalactiae (Group B Strep)
  7. 7Gram + cocci in pairs (lancet-shaped) Smooth, mucoid, alpha-hemolytic colonies on SBA Facultative anaerobe Oxidase - Catalase - Capsule Optochin (P disc) Susceptible Bile soluble +Streptococcus pneumoniae
  8. 8Gram + cocci Alpha hemolytic on SBA Facultative anaerobe Oxidase - Catalase - Optochin Resistant Bile esculin -Viridans Streptococci
  9. 9Gram + cocci, elongated Non-hemolytic, or alpha/beta on SBA Facultative anaerobe Oxidase - Catalase -/weak + PYR hydrolysis + Bile esculin + 6.5% NaCl + CAMP - Optochin ResistantEnterococcus spp.
  10. 10Gram + cocci, elongated Non-hemolytic, or alpha/beta on SBA Facultative anaerobe Oxidase - Catalase -/weak + Bile esculin + 6.5% NaCl - PYR hydrolysis - CAMP - Optochin ResistantGroup D Streptococci
  11. 11Gram + cocci Beta hemolytic on SBA Facultative anaerobe Oxidase - Catalase - Bile esculin -Non-group A, B, or D Streptococci
  12. 12Gram + cocci No hemolysis on SBA Facultative anaerobe Oxidase - Catalase - Bile esculin -Nonhemolytic Streptococci
  13. 13Gram + Rod, non-sporeforming Arranged in "palisades", "club-ends" Flat, dry, gray-white colonies on SBA (may have beta-hemolysis) Brown to black halo on Tinsdale agar Metachromatic granules w/ Loeffler's stain Catalase + Non-motile Bile esculin -Corynebacterium diptheriae
  14. 14Gram + Rod Pinpoint, white, nonhemolytic colonies Catalase + Glucose fermentation - Rapidly Urease +Corynebacterium urealyticum
  15. 15Gram + coccobacillus, non-sporeforming Catalase + Narrow zone of beta hemolysis on SBA Mobile at room temp, "umbrella type" Tumbling motility in hanging drop CAMP +, block type Hippurate hydrolysis + Bile esculin + VP & methyl red + 6.5% NaCl +Listeria monocytogenes
  16. 16Gram + slender rod, non-sporeforming Facultative anaerobe/microaerophilic Alpha hemolytic on SBA Nonmotile Catalase - H2S + VP - Bile esculin -Erysipelothrix rhusiopathiae
  17. 17Irregular gram + to gram variable rod Small colonies on SBA w/ narrow zone of beta hemolysis Catalase -Arcanobacterium
  18. 18Gram + rod "clue" cellsGardnerella vaginalis
  19. 19Gram + large, square-ended rods, "bamboo", sporeformer Raised, large, grayish-white, irregular edges, "beaten egg whites", no hemolysis on SBA Capsule Nonmotile Gelatin hydrolysis - Penicillin SusceptibleBacillus anthracis
  20. 20Gram + rod, sporeformer Beta hemolytic on SBA Motile Growth on PEA Gelatin hydrolysis + Penicillin ResistantBacillus cereus
  21. 21Gram + sporeformer Laboratory contaminantBacillus subtilis
  22. 22Aerobic Gram +, filamentous Weakly acid-fast Chalky, dry, crumbly, white colonies on BANocardia spp.
  23. 23Chalky, dry, crumbly, white colonies on BAStreptomyces spp.
  24. 24Diphtheroid looking Partially acid-fast Salmon color as colonies ageRhodococcus equi
  25. 25Gram - diplococci, non-sporeforming "kidney bean shaped" Aerobic Nonmotile Small, gray, translucent, raised colonies on Thayer-Martin agar Must be incubated with 3-5% CO2 Does not grow on SBA Oxidase + Catalase + Carbohydrate utilization: glucose onlyNeisseria gonorrhoeae
  26. 26Gram - diplococci, non-sporeforming Aerobic Nonmotile Capsule Oxidase + Catalase + Carbohydrate utilization: glucose & maltoseNeisseria meningitidis
  27. 27Gram - diplococci Oxidase + Catalase + DNAse + Non-fermentativeMoraxella catarrhalis
  28. 28Non-pathogenic Gram - diplococci Carbohydrate utilization: glucose, maltose, and lactoseNeisseria lactamica
  29. 29Gram - diplococci Non-pathogenic Carbohydrate utilization: glucose, maltose, sucrose Colonies are dry, wrinkled, breadcrumb-likeNeisseria sicca
  30. 30Gram - cocci Large mucoid colonies Reduce nitrite to nitrogen gas No pigmentsNeisseria mucosa
  31. 31Gram - rod Small pink, dry colonies on MAC Motile Ferments glucose & lactose TSIA A/A H2S - Indole + MR + VP - Citrate - SIM -++ Urea - LIA P/P H2S -Escherichia coli
  32. 32Gram - rod clear, colorless on MAC green on HE red on XLD Non-motile Non-lactose fermenter Urea - H2S - LIA P/Y TSIA K/A H2S - Citrate -Shigella dysenteriae
  33. 33Gram - rod clear, colorless on MAC green on HE red on XLD Non-motile lactose fermenter Urea - H2S - LIA P/Y TSIA K/A H2S - Citrate -Shigella sonnei
  34. 34Gram - rod Lactose fermenter IMViC --++ Nonmotile Capsule LIA P/P TSIA A/A H2S - SIM --- Urease weak +Klebsielle pneumoniae
  35. 35Gram - rod IMViC --++ Lactose fermenter MotileEnterobacter spp.
  36. 36Gram - rod ONPG + (slow lactose fermenter) DNase +Serratia spp.
  37. 37Gram - rod Swarming colonies Nonfermenter of lactose LIA R/Y (deaminate lysine) H2S + Citrate + Urea + SIM +-+Proteus mirabilis
  38. 38Gram - rod Nonfermenter of lactose LIA R/Y (deaminate lysine) TSIA K/A H2S - Citrate +Providencia spp.
  39. 39Gram - rod Nonfermenter of lactose LIA R/Y (deaminate lysine) Motile Urea + TSIA K/A H2S - Citrate -Morganella morganii
  40. 40Gram - rod Swarming colonies Nonfermenter of lactose LIA R/Y (deaminate lysine) H2S + Citrate + Urea + SIM +++ Sucrose fermenterProteus vulgaris
  41. 41Gram - rod Green w/ black center on HE Red w/ black center on XLD TSIA K/A H2S + Urea - SIM +-+ Citrate + LIA P/P H2S + PDA -Salmonella spp.
  42. 42Gram - rod H2S + May/may not ferment lactose Urea slow + LIA P/Y H2S + Citrate +Citrobacter spp.
  43. 43Gram - rod TSIA K/A H2S + PDA - Urea - Citrate + LIA K/K H2S + SIM +++Edwardsiella spp.
  44. 44Gram - rod Motile @ room temp Urea +Yersinia enterocolitica
  45. 45Gram - rod Urea + Motile @ room tempYersinia pseudotuberculosis
  46. 46Gram - short, plump rod Bipolar safety pin staining Non-lactose/non-sucrose fermenter H2S - PDA - Citrate - Urea - NonmotileYersinia pestis
  47. 47Gram - "curved" rod Facultative anaerobes Oxidase + Can reduce nitrate to nitrite O/129 disc susceptible String test + Ferments sucrose (yellow colonies on TCBS agar) Inositol -Vibrio cholerae
  48. 48Gram - "curved" rod Facultative anaerobes Oxidase + Can reduce nitrate to nitrite O/129 disc susceptible String test + Lactose fermenter Green colonies on TCBS (non-sucrose fermenter) Inositol -Vibrio vulnificus
  49. 49Gram - "curved" rod Facultative anaerobes Oxidase + Can reduce nitrate to nitrite O/129 disc susceptible String test + Halophilic (1-8%) Green colonies on TCBS (non-sucrose fermenter) Inositol -Vibrio parahaemolyticus
  50. 50Gram - "curved" rod Facultative anaerobes Oxidase + Can reduce nitrate to nitrite O/129 disc susceptible String test + Halophilic (up to 10%) Ferments sucrose (yellow colonies on TCBS agar) Inositol -Vibrio alginolyticus
  51. 51Gram - rod Oxidase + Glucose/lactose fermenter Pink colonies on modified CIN agar Resistant to O/129 disc 6.5% NaCl -Aeromonas
  52. 52Gram - rod Oxidase + Glucose/lactose fermenter Sensitive to O/129 disk Ferments inositol 6.5% NaCl -Plesiomonas
  53. 53Gram - rod Oxidizer/Oxidase + Motile Beta-hemolytic on SBA, flat spreading colonies w/ metallic sheen, fruity odor Growth at 42C Citrate + Blue pigmentPseudomonas aeruginosa
  54. 54Gram - rod Oxidase - Nonmotile Nitrate reduction - Purplish hue on MAC Glucose oxidizerAcinetobacter baumanii
  55. 55Gram - rod Plant pathogensBurkholderia cepacia
  56. 56Gram - rod Oxidase - Motile Catalase + Bile esculin - DNase + Gelatin hydrolysis +Stentrophomonas maltophilia
  57. 57Gram - rod Oxidase - Nonmotile Nitrate reduction - Purplish hue on MAC AssacharolyticAcinetobacter lwoffii
  58. 58Gram - rod Glander's disease Potential bioterror agentBurkholderia mallei
  59. 59Gram - rod Melioidosis Potential bioterror agent Nonfermentive Wrinkled colonies on SBABurkholderia pseudomallei
  60. 60Gram - rod Tan to brown pigment on SBA Oxidase + TSIA H2S +Shewanella spp.
  61. 61Gram - rod Oxidase + NonmotileMoraxella
  62. 62Small, paired, Gram - rod Oxidase + Nonmotile PAD + Non-oxidative Does not grow on MACOligella
  63. 63Small, pleomorphic gram - rod Require growth factors X (hemin) and V (NAD) Semi-opaque, gray-white, mucoid colonies on CHOC Satellitism w/ Staph on SBA Non-motile Facultative anaerobes Oxidase + Catalase + Reduce nitrates to nitritesHaemophilus influenzae
  64. 64Small, pleomorphic gram - rod, "school of fish" Require growth factors X (hemin) and V (NAD) Non-motile Facultative anaerobes Oxidase + Catalase + Reduce nitrates to nitritesHaemophilus ducreyi
  65. 65Small, pleomorphic gram - rod Require growth factor V (NAD) only Non-motile Facultative anaerobes Oxidase + Catalase + Reduce nitrates to nitritesHaemophilus parainfluenzae
  66. 66Gram - rod, pleomorphic Requires ferric iron and L-cysteine to grow Grayish-white, glistening, "ground glass" appearance on BCYELegionella pneumophila
  67. 67Small gram - rod/coccobacilli Obligate aerobe Smooth, glistening colonies, "half pearls", "mercury droplets" on Regan-Lowe agar No growth on MAC or SBA Catalase + Oxidase + Urease - Nitrate reduction - NonmotileBordatella pertussis
  68. 68Small gram - rod/coccobacilli Obligate aerobe Smooth, glistening colonies, "half pearls", "mercury droplets" on Regan-Lowe agar Growth on SBA, not on MAC Catalase + Oxidase - Urease + (24 hours) Nitrate reduction - NonmotileBordatella parapertussis
  69. 69Small gram - rod/coccobacilli Obligate aerobe Smooth, glistening colonies, "half pearls", "mercury droplets" on Regan-Lowe agar Growth on SBA & MAC Catalse + Oxidase + Urease + (4 hours) Nitrate reduction + MotileBordatella bronchiseptica
  70. 70Small gram - rods, "safety pin", bipolar staining Small, smooth, convex colonies on SBA or CHOC Musty odor No growth on MAC Oxidase +Pasteurella multocida
  71. 71Gram - coccobacilli Growth on SBA and CHOC Smooth, raised, translucent colonies Sheep/goat H2S - Urease variable Dye Inhibition Tionine/Fuschsin -/-Brucella melitensis
  72. 72Gram - coccobacilli Growth on SBA and CHOC Smooth, raised, translucent colonies Cattle CO2 may be required for growth Dye Inhibition Tionine/Fuschsin +/- H2S + Urease + (2hrs)Brucella abortus
  73. 73Gram - coccobacilli Growth on SBA and CHOC Smooth, raised, translucent colonies Pigs Dye Inhibition Tionine/Fuschsin -/+ H2S + Urease + (<0.5hrs)Brucella suis
  74. 74Gram - coccobacilli Growth on SBA and CHOC Smooth, raised, translucent colonies Dog Dye Inhibition Tionine/Fuschsin -/+ H2S - Urease + (<0.5 hrs)Brucella canis
  75. 75Gram -, pleomorphic, coccobacilli, "safety pin" Requires cysteine for growth Gray-white, smooth, raised colonies on CHOC Bioterror agentFrancisella tularensis
  76. 76Anaerobic gram + filamentous rod, "molar tooth" coloniesActinomyces israelii
  77. 77Anaerobic gram - pleomorphic Shapes range from fusiform to coccobacilliFusobacterium spp.
  78. 78Anaerobic gram + rod, sporeformer Double zone of hemolysis Pos lecithinase reaction of egg yolk agar Pos Reverse CAMP testClostridium perfringens
  79. 79Anaerobic gram - rod, small, pale-staining Grows on KVLB agar Grows on BBE agar (20% bile), forms black precipitateBacteroides fragilis
  80. 80Anaerobic gram - cocciVeillonella spp.
  81. 81Anaerobic gram - rod Pigmented, + fluorescence testPorphyromonas
  82. 82Anaerobic gram - rod Pigmented, + fluorescence testPrevotella
  83. 83Slender, slightly curved, rods Do not gram stain well Nonmotile Strict aerobe Slow growth Acid-fast bacilli Buff-colored, nonpigmented colonies Growth at 35-37 Niacin + Nitrate reduction + Catalase - PZA +Mycobacteria tuberculosis
  84. 84Slender, slightly curved, rods Do not gram stain well Nonmotile Strict aerobe Slow growth Acid-fast bacilli Nonpigmented Niacin - Nitrate reduction - T2H Growth - PZA -Mycobacterium bovis
  85. 85Slender, slightly curved, rods Do not gram stain well Nonmotile Strict aerobe Slow growth Acid-fast bacilli Smooth colonies Nonchromogenic, nonpigmented (yellow w/ age) Growth at 37 Niacin - T2H Growth + Catalase +Mycobacterium avium
  86. 86Slender, slightly curved, rods Do not gram stain well Nonmotile Strict aerobe Slow growth Acid-fast bacilli Growth at 37 Photochromogenic Strongly catalase + Tween hydrolysis + Nitrate reduction + PZA +Mycobacterium kansasii
  87. 87Slender, slightly curved, rods Do not gram stain well Nonmotile Strict aerobe Slow growth Acid-fast bacilli Photochromogenic Growth 28-32 Nitrate reduction - Catalase - Tween hydrolysis + Urease + PZA +Mycobacterium marinum
  88. 88Slender, slightly curved, rods Do not gram stain well Nonmotile Strict aerobe Slow growth Acid-fast bacilli Growth 25-37 Scotochromogenic Urease + Catalase + Tween hydrolysis - Nitrate reduction -Mycobacterium scrofulaceum
  89. 89Slender, slightly curved, rods Do not gram stain well Nonmotile Strict aerobe Slow growth Acid-fast bacilli Growth 30-33 Nonpigmented Catalase +Mycobacterium ulcerans
  90. 90Slender, slightly curved, rods Do not gram stain well Nonmotile Strict aerobe Fast growth Acid-fast bacilli 3-day arylsulfatase + Nitrate reduction +Mycobacterium fortuitum
  91. 91Slender, slightly curved, rods Do not gram stain well Nonmotile Strict aerobe Fast growth Acid-fast bacilli 3-day arylsulfatase + Nitrate reduction -Mycobacterium chelonae

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Flashcards on Microbiology-Bacteriology, created by Jaylene Brown on 31/03/2020.
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Jaylene Brown
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