Micro-B Lab Ch 3-4

Chrisitna Spangler
Flashcards by Chrisitna Spangler, updated more than 1 year ago
Chrisitna Spangler
Created by Chrisitna Spangler over 5 years ago


Staining Part 1 & 2

Resource summary

Question Answer
Polar Flagella A single flagellum at on end of a bacteria
Amphitrichous Flagella Flagellum at each end
Lophotrichous Flagella Several flagella emerge from one end
Pertrichous Flagella Flagella emerge from the entire cell
3 Steps to do a Smear prep. for staining 1. specimen is added to slide using water and then spread to a thin smear 2. Heat fix; kills microbes and adheres them to the slide 3. Stain: use dye to add contrast
How and with what tool do you inoculate a liquid (broth)? dip & swirl / using a loop
With what tool do you inoculate Agar plates? using a loop
How and with what tool do you inoculate solid/deeps? with a needle doing a stab
How and with what tool do you inoculate a solid / slant? with loop doing a fishtail
What temperature is 25°C relevant to? Room temp.
What temperature is 37°C relevant to? Human body temp.
Mixed Cultures contain 2 or more species of microbes in a single culture
Pure Cultures contain only a single species of microbe
Streak Plates Are often used to isolate microbes from mixed culture. Results in distinct colonies that arise from a single cell or colony-forming unit. (CFU)
Steam Sterilization High pressure steam used to remove all microbial life (including spores) from objects
Autoclaves Object that instruments or other items are put into in order to sterilize them. 121°C at 15 psi for 30 min.
What does TSA stand for ? trypticase soy agar
What is Serratia marcescens? A rod shaped Gram-neg bacteria that causes respiratory, UT infections, catheter related and wound infections among others.
What is Staphylococcus epidermidis? Gram positive and found in normal human flora, typically the skin, not usually pathogenic but can become so in hospital settings in immunocompromised patients
Culture A medium that contains living microbes
Aseptic technique A method of procedure that is performed in order to ensure that you, others or the environment are not contaminated.
What are Broths and why are they used? Broths are liquid media used to grow microbes when fresh cultures or large number of cells are required.
What are Agar slants and why are they used? Agar slants are used to grow stock cultures that can be refrigerated after incubation and maintained for several weeks.
What is differential media? Media that contains chemical indicators along with additional nutrients that may or may not be utilized by some species of microbes (metabolism of nutrient causes indicators to change color)
What is Selective media? Media that contain one or more inhibitory agents designed to prevent the growth of some microbes while favoring the growth of others.
What is General media? Permit the growth of all organisms regardless of type or nutritional peculiarities.
Escherichia coli Causes 80% of UTI's Use adhesins to attach to urinary epithelial Mobile and can travel along mucosal surfaces rod shaped, pathogenic, Gram-neg
Staphylococcus saprophyticus Causes UTI especially in younger women trasmitted from GI tract to urinary system and through sex, men can also get it through intercourse
What is the first symptom of a UTI caused by Staphylococcus saprophyticus? Often begins with Urethritis (inflammation of the urethra, burning sensation
If Urethritis from a UTI is not treated what can it lead to? Cystitis (infection of the urinary bladder)
--Pain in pelvic area (women) frequent urges to urinate --Cloudy urine or blood in urine --Pian in rectal region (men) are symptoms of... Cystitis
What is turbidity? A haze in fluid not clear or transparent because of stirred-up sediment or the like
Pyelonephritis A serious complication that is the result of untreated cystitis can lead to permanent kidney damage
What are the symptoms of Pyelonephritis? symptoms can mirror cystitis accompanied by back pain and high fever
What are 3 symptoms of Cystitis? 1. Pain in pelvic area women / rectal in men 2. Cloudy urine 3. Frequent urges to urinate
--Being female, sexually active, using diaphrams, completing menopause, using a catheter for urination and being immunocompromised are all risk factors for what? Urinary tract infection
What does the word ubiquitous mean? an organism likely can be isolated from soil, water, plants and animals to include (human)
Organisms that are not known to cause disease nonpathogenic
opportunistic pathogen pathogens capable of causing a disease state if introduced into a suitable part of the body.
vibrios a bacterial cell with a slightly curved morphology
pleomorphism When a variety of bacterial cell shapes can be seen in a given sample slender, elipsoidal or ovoid rods
PEA Indicator: Hemolytic activity beta hemolysis= complete degredation (+path) alfa hemolysis = incomplete degredation (protein metabolized/ heme is not) gamma hemolysis = NO degredation (0-path)
Phenylethyl Alcohol Agar PEA Selective agent: Pheylethyl Alcohol --Favors Gram + bacteria
Hemolytic Activity
Phenylethyl Alcohol Agar Selective agent: Pheynylethyl alcohol Favors Gram + bacteria
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