4974479
Description
Flashcards by Bee Brittain, updated more than 1 year ago
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Created by Bee Brittain
about 8 years ago
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| Question | Answer |
| Name 4 factors that effect enzyme action: | - Enzyme Concentration - Substrate Concentration - Temperature - pH |
| How does temperature increase the rate of reaction? | If the temperature increases, the substrate and enzyme molecules gain more kinetic energy, and so move around faster. This means there are more collisions, in turn meaning more enzyme-substrate complexes are formed in a given time, so the rate increases. |
| What happens past an enzymes optimum temperature and why? | Past an enzymes optimum temperature, it denatures. This is because there is so much energy it breaks the bonds that hold the enzymes shape. So, the active site changes shape and no more enzyme-substrate complexes are formed. |
| Draw the graph to show how temperature effects the rate of a reaction | |
| The pH of a solution is also a measure of what? | Its hydrogen ion concentration |
| What can happen above and below an enzymes optimum pH? Why is this? | Above and below the optimum pH, the OH- Ions and H+ ions can mess up the hydrogen and ionic bonds holding the enzymes tertiary structure in place |
| Draw a graph to show how pH effects enzyme action | |
| The higher the substrate concentration, the ______ the reaction. Explain your answer. | Faster. This is because there are more substrate molecules, so collisions are more likely and more active sites will be used = more enzyme-substrate complexes and a faster rate of reaction. |
| What is the saturation point? | When measuring substrate concentration, the saturation point is when all the enzyme's active sites are in use. This means the substrate concentration makes no difference from that point, on wards. |
| Over time, what happens to substrate concentration | It decreases |
| Draw a graph to show how substrate concentration effects enzyme activity | |
| What happens if enzyme concentration is increased in a reaction mixture? | Chances of a collision between a substrate and enzyme are more likely = more enzyme-substrate complexes. This means that increasing enzyme concentration increases the rate of reaction up to a certain point. |
| Increasing enzyme concentration does increase the rate of reaction, but what happens if the amount of substrate is limited? | If the amount of substrate is limited, the increase in enzyme concentration will increase the rate of reaction, but only up to a certain point and the afterwards it will have no further effect |
| Draw a graph to show the effect enzyme concentration has on the rate of a reaction |
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