Flashcards
Dry Reagent Chemistry
Cards in this deck (16)
- 1What is dry reagent chemistry?quantitative analysis of analyte using dry reagents
- 2what was one of the first dry chemistry tests?the acid test using litmus paper
- 3what is the basic structure of Dry Reagent Film?1) Upper slide mount
2) Spreading Layer
3) Analytical Layer (one or more)
4) Support
5) Lower slide mount - 4what is the spreading layer for?1) rapid, uniform diffusion of sample
2) evenly spread sample onto analytical layers
3) made of fibrous material/membrane - 5what is the analytical layer(s) for?1) incorporates one or more reagents
2) thin, porous gelatin or fibrous material
3) Fibrous material can: separate RBCs from serum, separate reaction products
4) one reaction per layer (each layer is dried before the next layer is applied) - 6what does the reflective layer do?1) At bottom of analytical layer
2) reflects light emitted by reagent layers
3) can be a pigment (TiO2) or material (metal foil) - 7what is the support layer?1) plastic (transmits lights)
2) can include a reflective layer - 8How can dry chemistry be use clinically?1) determine diabetics (glucose detection)
2) Arthritis (CRP detection)
3) Hypothyroidism (serum T4) - 9What reagents are used in the detection of a diabetic patient?In the Analytical layer - 1) glucose oxidase
2) buffer pH5
3) peroxidase
4) dye precursor (leuco dye) - 10how do you know if the patient is diabetic?the dye turns red
- 11how are RBCs removed from the blood sample?analytical layer includes fibrous material that separates RBCs form serum
- 12What is in the reflectance layer of analytical layer for glucose test?serum creatinine
layer 1 - buffer + creatinine amidohydrolase (converts creatinine to creatine and produced ammonia)
layer 2 - bromophenol blue (changed colour with ammonia) - 13what does H2O2 do in these reactions?produces a colour when reacted with leuco dye in the presence of peroxidase
- 14How would you test for Rheumatoid arthritis?Dry reagent chemistry using C-reactve protien (CRP)
- 15why is CRP used?it is a marker of inflammation
- 16what are the reagents used for CRP test?In spreading layer?
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- Hons Clinical Biochem Flashcards on Dry Reagent Chemistry , created by Alice Brealy on 25/04/2016.
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- Alice Brealy
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