535548
Description
Flashcards by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312
almost 12 years ago
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| Question | Answer |
| Method of Transfer | Movement of DNA by direct cell to cell contact |
| Recipient of DNA | Exconjugant |
| Form of DNA Transferred | Usually a plasmid, or as conjugated transposons (these must integrate into chromosomes) |
| How do plasmids pick up new genes? | Transposition (genes capable of independent replication) or excision from/insertion into the chromosome |
| Stage 1 of Conjugation | Surface Adhesions (Gram +) or Conjugative Pili (G-) mediate donor-cell interactions |
| Stage 2 of Conjugation | Mating Pair Junction is formed |
| Stage 3 of Conjugation | DNA (usually ss) is transferred |
| Stage 4 of Conjugation | The recipient synthesizes a complimentary strand to the ss DNA gained, as does the donor |
| Transfer Apparatus- Relaxosome | Contains OriT & Relaxase |
| Transfer Apparatus - Role of Relaxase | Cleaves DNA at OriT & binds to 5' of transferring strand |
| Transfer Apparatus- Type IV Secretion System | Encoded by mpf genes, it recognises Relaxase, not DNA |
| Transfer Apparatus- Connective Coupling Protein | Connects Relaxosome & T4SS |
| Key Conjugative Plasmid Genes- Stability Genes | Control segregation |
| Key Conjugative Plasmid Genes- Replication genes | Co-ordinate replication with host genome (so it replicates once per cell cycle) |
| Key Conjugative Plasmid Genes- Propogation genes | Ensure correct transfer between cells |
| Key Conjugative Plasmid Genes- Adaptation gene | The genes that confer the advantage |
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