Conjugation

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Flashcards by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312 almost 6 years ago
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Medical Microbiology (Gene Transfer) Flashcards on Conjugation, created by gina_evans0312 on 02/08/2014.

Resource summary

Question Answer
Method of Transfer Movement of DNA by direct cell to cell contact
Recipient of DNA Exconjugant
Form of DNA Transferred Usually a plasmid, or as conjugated transposons (these must integrate into chromosomes)
How do plasmids pick up new genes? Transposition (genes capable of independent replication) or excision from/insertion into the chromosome
Stage 1 of Conjugation Surface Adhesions (Gram +) or Conjugative Pili (G-) mediate donor-cell interactions
Stage 2 of Conjugation Mating Pair Junction is formed
Stage 3 of Conjugation DNA (usually ss) is transferred
Stage 4 of Conjugation The recipient synthesizes a complimentary strand to the ss DNA gained, as does the donor
Transfer Apparatus- Relaxosome Contains OriT & Relaxase
Transfer Apparatus - Role of Relaxase Cleaves DNA at OriT & binds to 5' of transferring strand
Transfer Apparatus- Type IV Secretion System Encoded by mpf genes, it recognises Relaxase, not DNA
Transfer Apparatus- Connective Coupling Protein Connects Relaxosome & T4SS
Key Conjugative Plasmid Genes- Stability Genes Control segregation
Key Conjugative Plasmid Genes- Replication genes Co-ordinate replication with host genome (so it replicates once per cell cycle)
Key Conjugative Plasmid Genes- Propogation genes Ensure correct transfer between cells
Key Conjugative Plasmid Genes- Adaptation gene The genes that confer the advantage
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