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Flowchart

DISAGGREGATION OF PLANTS

Rashedha Camila English 4.5Average rating 4.5 out of 5 (2 ratings)

Flowchart content

  1. DISAGGREGATION OF PLANTS

  2. PRIMARY AGGREGATION

  3. MECHANICAL DISAGGREGATION

  4. ENZYMATIC DISAGGREGATION

  5. ​​​​DISSECTION

  6. FINE CHOPPING

  7. WASH BY SETTLING

  8. TRANSFER IN LAST WASH

  9. REMOVE BSS

  10. ADD THIN FILM OF MEDIUM

  11. INCUBATE FOR 2-3 WEEKS

  12. EXPLANT OUTGROWTHS

  13. TISSUE SAMPLE

  14. CELL TISSUE + DEBRIS

  15. MORE CELLS + TISSUE DEBRIS

  16. VIABLE CELLS + TISSUE DEBRIS

  17. VIABLE CELLS + DAMAGED CELLS

  18. PRIMARY ADHERENT CELL CULTURE

  19. BIOPSY IN MEDIUM + ANTIBIOTIC

  20. DISSECTION IN BSS + ANTIBIOTIC

  21. CHOPPED TISSUE

  22. COMPLETE MEDIUM + COLLAGENASE

  23. DISAGGREGATED TISSUE + TISSUE RESIDUES

  24. DISPERSED CELLS + RESIDUAL CULTURE OF EPITHELIAL ELLS

  25. DISPERSED CELL SUSPENSION CONTAINS ENRICHED FIBROBLASTS

  26. PRIMARY CELL CULTURE

  27. ENRICHED EPITHELIAL FRACTION

  28. PRIMARY CELL CULTURE

  29. EDTA TREATMENT

  30. COLLAGENSE

  31. CENTRIFUGE

  32. RESUSPEND

  33. WASH

  34. PRIMARY CELL CULTURE

Information

Description
Flowchart on DISAGGREGATION OF PLANTS, created by Rashedha Camila on 27/12/2017.
Created by
Rashedha Camila · Dec 2017
Updated
Jan 3, 2018
Views
31
Rating
Average rating 4.5 out of 5 (2 ratings)

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