Flowchart
diaggregation of explants
Flowchart: Diaggregation of explants
Flowchart content
Selection and isolation of organ
Dissection and removal of necrotic cells and fat cells
Mechanical dissagregation
Enzymatic dissagregation
Primary expant technique
Chopping and slicing of tissue, sieving, syringing, pippeting, etc.
Trypsin
Collagenase
Other enzymes
( bacterial proteases)
Explant
Warm Trypsinization
Cold Trypsinization
Resuspended and seed
Primary culture
sub-culture
Cell line
Information
- Description
- Flowchart on diaggregation of explants, created by Isabell Rupa on 03/01/2018.
- Created by
- Isabell Rupa
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