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Flowchart

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JOSELYNE TEH English

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  1. Prepare Slide

  2. Deparaffination

  3. Xylene (3 changes for 5 minutes each)

  4. Rehydration

  5. Hydrate sections through graded alcohol

  6. Wash in deionized water for 1 minute

  7. Nuclear stain (Hematoxylin 15 minutes)

  8. Tap water

  9. Differentiation (1% acid alcohol; 1-3 rapid dips)

  10. Tap water

  11. Bluing (Ammonia water; 10-15 dips)

  12. Running water

  13. Counterstain (Eosin)

  14. Dehydration (95% Ethyl Alcohol; 15-20 dips)

  15. Dehydration (95% Ethyl Alcohol; 15-20 dips)

  16. Dehydration (95% Ethyl Alcohol; 15-20 dips)

  17. Dehydration (95% Ethyl Alcohol; 15-20 drops)

  18. Clearing (Xylene; 15-20 dips)

  19. Clearing (Xylene; 15-20 dips)

  20. Clearing (Xylene; 15-20 dips)

  21. Air dry

  22. Mount

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Flowchart on Untitled, created by JOSELYNE TEH on 16/05/2022.
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JOSELYNE TEH
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