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1061694
Determinación de fosfato en extractos lipídicos
Description
Mind Map on Determinación de fosfato en extractos lipídicos, created by Tatiana Romero G on 09/07/2014.
Mind Map by
Tatiana Romero G
, updated more than 1 year ago
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Created by
Tatiana Romero G
almost 10 years ago
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Resource summary
Determinación de fosfato en extractos lipídicos
Muestras
Mezclas HT y ST
Hacer diluciones 1:10
Mezclas HH y SH
Hacer diluciones 1:10
Curva Estandar
Tomar alicuotas de 10 a 100 uL
Fosfato diacido de Potasio 0.8 mM
Diluir hasta un volumen final de 100 uL
Agua MiliQ
50 uL de Nitrato de Mg al 10%
en etanol al 95%
Calentar a sequedad
300 uL HCl 1N
Incubar
37°C 15 min
700 uL mezcla Ac. Ascorbico 10% - Molibdato de Amonio
Proporción 1:6
Incubar
37°C 1 hora
Leer en el espectrofotómetro a 820 nm
Tomar alicuotas de 20 a 40 uL
Tomar alicuotas de 10 a 50 uL
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