changing the temperature changes the
rate of an enzyme- catalysed reaction
a higher temperature increases the rate at first
more heat means the enzymes
and the substrate particles have
more energy - making the
enzymes and the substrate
particles move about more -
more likely to meet up and react
- higher collison rate
low temperatures have the opposite
effect - lower collision rate and slower
reaction
if it gets too hot, some of the bonds holding the
enzyme together will break
making the enzyme lose its shape - active site
doesn't fit with the substrate
reaction can't catalysed and stops - enzyme
doesn't function
enzymes like the right pH too
pH has an effect on the enzymes
pH is too high or too low - interferes
with the bonds holding the enzymes
together
changing the shape of the active site and
substrate
enzyme = denatured = irreversible
all enzymes have an optimum pH that they work best at
often neutral pH7
each enzyme has its own optimum
temperature when the reaction is fastest
temp before it gets too hot and starts to denature
uman optimum temp = 37
Q10 values show how rate of reaction changes with temp
how much the rate changes when the temp is raised by 10`c