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Quantification of Protein: Bradford Method
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Quantification of Protein: Bradford Method
Binding of Coomassie Blue G250 to Protein.
Acidic Conditions
Results
- Anion absorbance: 590 nm
- Cation Absorbance: 470 nm
If absorption spectrum overlap, assay responds non-linearly in standard curve.
Dye most readily binds to Lysine & Arginine.
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- Chemical Biology and Enzyme (Chemical Biology) Mind Map on Quantification of Protein: Bradford Method, created by Clevanisty Clandisity on 05/12/2019.
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- Clevanisty Clandisity
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