AS Biology - Cell Structure - 2.1.1

AS Biology - Cell Structure - 2.1.1

1. Microscopy

   • Light Microscope
     • Max Mag: X2000 Max Res: 200nm
     • Small, portable, inexpensive, natural colour, live specimens
     • Staining
       • Visualise components better
       • Increase contrast
       • Different stains, stain different
   • Transmission Electron
     • Max Mag: X500,000 Max Res: 0.5nm
     • Very expensive, large, not portable, dead specimens, 2D B&W
   • Laser Scanning Confocal
     • Max Mag: X2000
     • 3D, shows different depths
   • Scanning Electron
     • Max Mag: X500,000 Max Res: 5nm
     • Expensive, large, specimen dead, B&W 3D

2. Magnification Formula

   • "IAM"
   • Magnification: how many X bigger than actual specimen
   • Resolution: Distinguish 2 points as separate
   • Image length
   • Actual length
   • MM - (x1000) - UM - (x1000) - NM
   • Magnification

3. Eukaryotic Cells

4. Prokaryotic Cells

   • Single Celled

5. Cytoskeleton