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Molecular Motors- Helicases

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Structural Basis for Biological Function (Molecular Motors) Quiz sobre Molecular Motors- Helicases, criado por gina_evans0312 em 15-12-2013.
gina_evans0312
Quiz por gina_evans0312, atualizado more than 1 year ago
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Criado por gina_evans0312 mais de 10 anos atrás
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Resumo de Recurso

Questão 1

Questão
DNA helicase unwinds DNA into ssDNA for what processes?
Responda
  • DNA repair
  • DNA replication
  • DNA recombination
  • DNA destruction

Questão 2

Questão
DNA helicase's process and translocate branched structures
Responda
  • True
  • False

Questão 3

Questão
Name (a) branched DNA structure(s)
Responda
  • Holliday junctions
  • D loops
  • Binary helix
  • T loops

Questão 4

Questão
RNA helicases cannot destabilize RNA secondary structures
Responda
  • True
  • False

Questão 5

Questão
RNA helicases help in which of the following processes A- Promote ribosome assembly B- RNA translocation C-RNA splicing D- RNA editing and transport E-RNA degredation
Responda
  • A, C & D
  • B, C & E
  • A only
  • E only
  • All of them

Questão 6

Questão
Some helicases can work on both RNA & DNA
Responda
  • True
  • False

Questão 7

Questão
Which of the following unwind RNA-DNA hybrids
Responda
  • RNA helicase, then DNA helicase
  • DNA helicase, then RNA helicase
  • RNA/DNA helicases

Questão 8

Questão
RNA/DNA helicases perform which of the following functions?
Responda
  • Transcription termination
  • Regulation of DNA replication initiation
  • RNA degredation

Questão 9

Questão
All helicases use energy from NTP hydrolysis to translocate along polynucleotides
Responda
  • True
  • False

Questão 10

Questão
Helicases are dependent on Fe2+
Responda
  • True
  • False

Questão 11

Questão
Helicases can take which of the following forms (commonly)
Responda
  • Hexamers
  • Monomers
  • Trimers
  • Dimers
  • Tetramers
  • Pentamers

Questão 12

Questão
Helicases can have an oligomeric structure
Responda
  • True
  • False

Questão 13

Questão
Helicases can be
Responda
  • Unidirectional
  • Bidirectional
  • Both
  • Bidirectional or unidirectional

Questão 14

Questão
What is the difference between translocases and helicases?
Responda
  • Translocases -transcribe and separate DNA strands Helicases- transcribed DNA strands only
  • Helicases-transcribe and separate DNA strands Translocases- transcribed DNA strands only

Questão 15

Questão
Give an example of a DNA/RNA unwinding helicase/translocase
Responda
  • PCRA
  • Type 1 restriction enzymes
  • Ruv B/Reg G
  • Mfd

Questão 16

Questão
Name a helicase/translocase that effects protein motion on DNA
Responda
  • PCRA
  • Type 1 Restriction Enzyme
  • Ruv B/ Reg G
  • Mfd

Questão 17

Questão
Name a necleoprotein complex remodelling helicase
Responda
  • Mfd
  • Ruv B/Reg G
  • PCRA
  • Type 1 Restriction Enzymes

Questão 18

Questão
Name a DNA/RNA remodelling helicase
Responda
  • Ruv B/ Reg G
  • PCRA
  • Type 1 Restriction Enzymes
  • Mfd

Questão 19

Questão
Helicases are divided into seven superfamilies based on primary structure, and then 3 structural classes
Responda
  • True
  • False

Questão 20

Questão
The two structural classes are monomers and dimers
Responda
  • True
  • False

Questão 21

Questão
All helicases contain a Rec B fold
Responda
  • True
  • False

Questão 22

Questão
EVERY helicase molecule contains which of the following?
Responda
  • An ATP binding site
  • An NTP binding site
  • A separate polynucleotide binding site
  • A GTP binding site
  • A linked polynucleotide binding site

Questão 23

Questão
The _TP binding site and the nucleotide binding site on a helicase are allosterically linked
Responda
  • True
  • False

Questão 24

Questão
Type A helicases (like PCRA) travel in which direction?
Responda
  • 3' > 5'
  • 5' > 3'
  • Both ways

Questão 25

Questão
Rec D is a Type 2 helicase- in which direction does it travel?
Responda
  • 5' > 3'
  • Both ways
  • 3' . 5'

Questão 26

Questão
Rec BCD is a bipolar helicase
Responda
  • True
  • False

Questão 27

Questão
In a bacterial Holliday Junction with a stalled replication fork, what is required to allow replication to continue?
Responda
  • RuvB
  • ATP
  • PRCA
  • GTP

Questão 28

Questão
If Holliday Junctions cannot be repaired, they lead to a cruciform structure
Responda
  • True
  • False

Questão 29

Questão
What is the slowest rate of movement of a helicase?
Responda
  • 10 bp/sec
  • 8 bp/sec
  • 12 bp/sec

Questão 30

Questão
Rate of helicase movement is dramatically dependent on protein partners
Responda
  • True
  • False

Questão 31

Questão
The fastest known helicase moves around 4000 bp/sec
Responda
  • True
  • False

Questão 32

Questão
Define Helicase Processivity
Responda
  • How fast it moves along the DNA/RNA
  • How far it can move before it falls off
  • How far it moves per substrate hydrolysis

Questão 33

Questão
Helicases can move around 10-30,000 base pairs before falling off depending on the helicase (and it's protein partners)
Responda
  • True
  • False

Questão 34

Questão
Step size is a theoretical limit due to the entropy of NTP hydrolysis
Responda
  • True
  • False

Questão 35

Questão
Step size is the theoretical avg no of bp unwound per NTP hydrolysis cycle
Responda
  • True
  • False

Questão 36

Questão
The limit on step size = Energy required to melt the bp x step size
Responda
  • True
  • False

Questão 37

Questão
What is the experimental limit of step size?
Responda
  • 0.3-3 bp
  • 0.5- 5bp
  • 0.7- 7 bp

Questão 38

Questão
What is this image showing?
Image:
Inchworm_Movement_Model (image/png)
Responda
  • The 'Inchworm' helicase movement model
  • The 'active rolling model'

Questão 39

Questão
Which of the following is believed to be the unwinding mechanism used by helicases?
Responda
  • Passive- DNA is frayed by heat and the translocation occurs in the presence of ATP
  • Active- ATP activates molecules that bind to and actively distort DNA before ATP dependent translocation can occur

Questão 40

Questão
PCRA is made of how many domains?
Responda
  • 4 - 1A/B & 2A/B
  • 2 - 1A & 2A
  • 6 1A/B, 2A/B & 3A/B

Questão 41

Questão
PCRA activity is dependent on its protein partners
Responda
  • True
  • False

Questão 42

Questão
PCRA is an ATPase with...
Responda
  • A 3' > 5' polarity
  • A 5' > 3' polarity
  • A low rate of 30 bp/sec
  • A high rate of 300 bp/sec
  • A low processivity of 10 bp/sec
  • A high processivity of 100 bp/sec
  • A step size of 16 bp/ATP
  • A step size of 6 bp/ATP

Questão 43

Questão
The conserved Helicase motifs of PCRA are in the accessory domains
Responda
  • True
  • False

Questão 44

Questão
Aromatic stacking occurs between the inserted aa and bp
Responda
  • True
  • False

Questão 45

Questão
Which of the following will bind to the DNA
Responda
  • Tryptophan
  • Phenylalanine
  • Tyrosine
  • Histidine

Questão 46

Questão
The complex of PCRA-DNA remains the same during ATP binding and ATP hydrolysis
Responda
  • True
  • False

Questão 47

Questão
Duplex distortion in the PCRA substrate complex suggests a Passive unwinding system
Responda
  • True
  • False

Questão 48

Questão
Partner proteins are required by PCRA for which processes?
Responda
  • Loading
  • Unwinding
  • Activation
  • Catalytic modification

Questão 49

Questão
Hexameric helicases can be part of the replisome
Responda
  • True
  • False

Questão 50

Questão
Hexameric helicases have a toroidal strcture to confer high processivity
Responda
  • True
  • False

Questão 51

Questão
ATP binds where on hexameric helicases?
Responda
  • To the pockets present in each monomer
  • To the pocket made by the interaction of all 6 monomers

Questão 52

Questão
ssDNA is bound in the central channel to a 'spiral staircase' of bining loops
Responda
  • True
  • False

Questão 53

Questão
ATP binding pocket changes _ around the ring
Responda
  • Sequentially- one at a time
  • At the same time
  • Sequentially- two at a time

Questão 54

Questão
ATP binding correlates with loop hight in the central channel
Responda
  • True
  • False

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