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Chapter 3 Observing Microorganisms through a Microscope
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Questions in this quiz (55)
1Which of the following is NOT equal to 1 mm?
- A0.001m
- B106nm
- C0.1cm
- D100m
- E10-3m
2What structure does light pass through after leaving the condenser in a compound light microscope?
- Aocular lens
- Bobjective lens
- Cspecimen
- Dilluminator
3Which of the following pairs is mismatched?
- Anigrosin negative stain
- Bmethylene blue simple stain
- Cacidic dye capsule stain
- Dbasic dye negative stain
- Ecrystal violet simple stain
4Which of the following places the steps of the Gram stain in the correct order?
1-Alcohol-acetone
2-Crystal violet
3-Safranin
4-Iodine- A1-2-3-4
- B2-1-4-3
- C2-4-1-3
- D4-3-2-1
- E1-3-2-4
5Which of the following pairs is mismatched?
- Aalcohol-acetone decolorizer
- Bcrystal violet basic dye
- Csafranin acid dye
- Diodine mordant
- Ecarbolfuchsin basic dye
6Which of the following is NOT true regarding the acid-fast stain?
- AIt is used to identify members of the genus Mycobacterium.
- BAcid-fast cells retain the primary dye after treatment with acid-alcohol.
- CIf cells are acid-fast, they are gram-negative.
- DAcid-fast cells appear red in a completed acid-fast stain.
- ENon-acid-fast microbes appear blue in a completed acid-fast stain.
7The purpose of a mordant in the Gram stain is to
- Aremove the simple stain.
- Bmake the bacterial cells larger.
- Cmake the flagella visible.
- Dprevent the crystal violet from leaving the cells.
- Emake gram-negative cells visible.
8Which of the following places the steps in the correct sequence?
1-Staining
2-Making a smear
3-Fixing- A1-2-3
- B3-2-1
- C2-3-1
- D1-3-2
- Ethe order is unimportant
9The negative stain is used to
- Avisualize endospores.
- Bdetermine Gram reaction.
- Cdetermine flagella arrangement.
- Dvisualize capsules.
- Edetermine cell size.
10Simple staining is often necessary to improve contrast in which microscope?
- Acompound light microscope
- Bphase-contrast microscope
- Cdarkfield microscope
- Dfluorescence microscope
- Eelectron microscope
11Which microscope is used to see internal structures of cells in a natural state?
- Acompound light microscope
- Bphase-contrast microscope
- Cdarkfield microscope
- Dfluorescence microscope
- Eelectron microscope
12Which microscope uses visible light?
- Aconfocal microscope
- Bdifferential interference contrast microscope
- Cfluorescence microscope
- Dscanning acoustic microscope
- Escanning electron microscope
13Which microscope achieves the highest magnification and greatest resolution?
- Acompound light microscope
- Bphase-contrast microscope
- Cdarkfield microscope
- Dfluorescence microscope
- Eelectron microscope
14 In using this microscope, the observer does NOT look directly at an image through a lens.
- Acompound light microscope
- Bphase-contrast microscope
- Cdarkfield microscope
- Dfluorescence microscope
- Eelectron microscope
15This microscope produces an image of a light cell against a dark background; internal structures are NOT visible.
- Acompound light microscope
- Bphase-contrast microscope
- Cdarkfield microscope
- Dfluorescence microscope
- Eelectron microscope
16A virus measures 100 nm in length. What is its length in m?
- A10m
- B1m
- C0.1m
- D0.01m
- E0.001m
17 Which of the following is never useful for observing living cells?
- Aphase-contrast microscope
- Bdarkfield microscope
- Cscanning acoustic microscope
- Dscanning electron microscope
- Ebrightfield microscope
18A microorganism measures 5 m in length. Its length in mm would be
- A500mm
- B50mm
- C0.5mm
- D0.05mm
- E0.005mm
19Which of the following correctly traces the path of light through the compound microscope?
- Alight source; condenser; specimen; objective lens; ocular lens
- Bcondenser; light source; specimen; ocular lens; objective lens
- Clight source; specimen; condenser; objective lens; ocular lens
- Dcondenser; light source; specimen; objective lens; ocular lens
- Elight source; condenser; objective lens; specimen; ocular lens
20In Figure 3.1, line c points to the microscopes
- Ailluminator
- Bcondensor
- Cocular lens
- Dobjective lens
21Which microscope can be used to visualize DNA or botulinum toxin?
- Acompound light microscope
- Bphase-contrast microscope
- Cscanning tunneling microscope
- Dconfocal microscope
- Escanning electron microscope
22Which microscope is used to observe a specimen that emits light when illuminated with an ultraviolet light?
- Acompound light microscope
- Bphase-contrast microscope
- Cdarkfield microscope
- Dfluorescence microscope
- Eelectron microscope
23Which microscope is most useful for visualizing a biofilm?
- Acompound light microscope
- Bphase-contrast microscope
- Cfluorescence microscope
- Dscanning acoustic microscope
- Etransmission electron microscope
24Which microscope takes advantage of differences in the refractive indexes of cell structures?
- Acompound light microscope
- Bphase-contrast microscope
- Cdarkfield microscope
- Dfluorescence microscope
- Eelectron microscope
25You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the first dye. What is the appearance of the bacteria at this point?
- Apurple
- Bred
- Ccolorless
- Dbrown
26You are performing a Gram stain on gram-negative bacteria and you stop after the addition of the mordant. What is the appearance of the bacteria at this point?
- Apurple
- Bred
- Ccolorless
- Dbrown
27You are performing a Gram stain on gram-negative bacteria and you stop after the decolorizer step. What is the appearance of the bacteria at this point?
- Apurple
- Bred
- Ccolorless
- Dbrown
28 You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the counterstain. What is the appearance of the bacteria at this point?
- Apurple
- Bred
- Ccolorless
- Dbrown
29Which type of stain is most useful in helping clinicians to decide which antibiotic to prescribe for a bacterial infection?
- Anegative stain
- Bsimple stain
- Cgram stain
- Dendospore stain
- Eflagella stain
30Which of the following pairs is mismatched?
- Aconfocal microscope produces a three-dimensional image
- Bdarkfield microscope uses visible light
- Cfluorescence microscope uses a fluorescent light
- Dscanning electron microscope produces a three-dimensional image
- Escanning tunneling microscope allows visualization of atoms
31 What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective lens?
- A4.5x
- B10x
- C45x
- D100x
- E450x
32You are studying a cell structure that is approximately 100 nm in size. Which of the following provides the greatest magnification you can use to see this structure?
- Abrightfield microscope
- Bdarkfield microscope
- Ctransmission electron microscope
- Dphase-contrast microscope
- Escanning electron microscope
33Which microscope uses two beams of light to produce a three-dimensional color image?
- Afluorescence microscope
- Bphase-contrast microscope
- Cdarkfield microscope
- DDIC microscope
- Eelectron microscope
34Which microscope is used to see intracellular detail in a living cell?
- Afluorescence microscope
- Btwo-photon microscope
- Catomic force microscope
- Dtransmission electron microscope
- Ebrightfield microscope
35Which microscope is used to observe viruses and the internal structure of thinly sectioned cells?
- Atransmission electron microscope
- Bdarkfield microscope
- Cfluorescence microscope
- Dbrightfield microscope
- Escanning electron microscope
36Which microscope is used to see detail of a 300-nm virus?
- Afluorescence microscope
- Bphase-contrast microscope
- Cdarkfield microscope
- DDIC microscope
- Eelectron microscope
37Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures are
- Acell walls
- Bcapsules
- Cendospores
- Dflagella
- Ethe answer cannot be determined
38 Which step in the Gram stain is the critical step in differentiating gram-positive cells from gram-negative cells?
- Asafranin
- Balcohol-acetone
- Ciodine
- Dcrystal violet
39 You find colorless areas in cells in a Gram-stained smear. What should you apply next?
- Aan acid-fast stain
- Ba flagella stain
- Can endospore stain
- Da simple stain
40Which microscope is best used for observing the surfaces of intact cells and viruses?
- Aphase-contrast microscope
- Bdarkfield microscope
- Cfluorescence microscope
- Dbrightfield microscope
- Escanning electron microscope
41Bacterial smears are fixed before staining to
- Aaffix the cells to the slide.
- Bmake their walls permeable.
- Caccept stain.
- Dmake the cells visible.
42The resolution of a microscope can be improved by changing the
- Acondenser
- Bfine adjustment
- Cwavelength of light
- Ddiaphragm
- Ecoarse adjustment
43 A student is looking at a bacterial specimen using the oil immersion lens, but has forgotten to put immersion oil on the slide. The specimen will appear
- Asmaller than it would if immersion oil was used.
- Blarger than it would if immersion oil was used.
- Csomewhat fuzzy and have poor resolution.
- Dthe same as it would if the immersion oil was used.
- Eto have no color.
44The purpose of the ocular lens is to
- Aimprove resolution.
- Bmagnify the image from the objective lens.
- Cdecrease the refractive index.
- Dincrease the light
- Edecrease the light
45In microscopy, the term resolution
- Arefers to the ability to distinguish fine structure and detail in a specimen.
- Bis the same as the total magnification of a specimen.
- Cis improved when longer wavelengths of light are employed.
- Dis only observed in stained specimens.
- Erefers to magnification when using the electron microscope.
46The counterstain used in the Gram stain is a basic dye.
- ATrue
- BFalse
47In a completed Gram stain, gram-negative bacteria are colorless.
- ATrue
- BFalse
48In a completed Gram stain, gram-positive bacteria are purple.
- ATrue
- BFalse
49If acid-fast bacteria are stained with the Gram stain, they will stain gram-negative.
- ATrue
- BFalse
50The limit of resolution of the compound microscope illuminated with visible light is approximately 0.2 m.
- ATrue
- BFalse
51Both phase-contrast microscopy and differential interference contrast microscopy are used to
view the internal structures of cells without staining.- ATrue
- BFalse
52Cells viewed in darkfield microscopy appear similar to those stained with the negative stain.
- ATrue
- BFalse
53The capsules and flagella of bacteria can be observed in gram-stained smears.
- ATrue
- BFalse
54The greater resolution of the electron microscope compared to the compound microscope is due to the longer wavelengths of the electrons used to examine specimens.
- ATrue
- BFalse
55Scanned probe microscopy is used to examine fine detail of molecular complexes, such as blood clots, or molecules, such as DNA.
- ATrue
- BFalse
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- Science Quiz on Chapter 3 Observing Microorganisms through a Microscope , created by Roxana Caceres on 17/09/2017.
- Author
- Roxana Caceres
- Language
- English
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