Quiz
Wk 8 - Foundations of Anatomical Pathology
Questions in this quiz (16)
1Define Histopathology
- Athe study of changes in tissues caused by disease.
- Bthe study of changes in cells caused by disease.
2Select Three hazards associated with working in a Histopathology laboratory.
- A- Chemical: fixatives, stains, chemicals
- B– Biological: cross infection
- C– Physical: sharps, radiation
- D- Safety: slips, trips, falls
3Explain what a fixative does
- Apreservation of tissue specimen
- Bdecontaminates all viruses associated with the tissue specimen
4List Six features of an ideal fixative
- A• Will not swell or shrink tissue
- B• Is economical
- C• Has convenient shelf life and storage
- D• Will be safe for user and environment
- E• Will preserve tissue in life like manner
- F• Will not add artefact material to tissue
- G• Will add artefact material to tissue
5Select Three fixative/s that can be used for Cytology smears?
- ASpray fixed with aerosol of propanol and acetone+ Poly ethylene glycol- a water soluble wax
- BMethanol
- CEthanol
- DXylene
- ESodium dihydrogen phosphate monohydrate 4g
6Select Three physical agents that can be used to assist in the fixation of tissues.
- AMicrowave
- BHeat
- CUltrasound
- DFreeze
7True or false: autolysis represents the changes to tissue that occur before fixation due to bacterial contamination.
- ATrue
- BFalse
8What type of fixative is Neutral Buffered Formalin classed as?
- AUniversal fixative
- BFixative used for specific purposes
9Why is Formalin Buffered to a neutral pH?
- Abecause the further a fixative is from the pH of body tissues the more distortion you can get in the tissue at the cellular level.
- Bbecause the further a fixative is from the pH of body tissues the less distortion you can get in the tissue at the cellular level.
10What is the function of xylene in tissue processing?
- ATo cut sections, the tissue has to be embedded in paraffin wax, but wax is not soluble in water or alcohol. However, it is soluble in a paraffin solvent called 'xylene'.
- BCleaning up the lab station to prevent contamination of other specimens and users of the facilities
11Why is the histological section cut to approximately 5µm thick?
- A- Sample needs to be thin enough for light microscopy (about 1 cell layer thick)
- makes it easier to evaluate tissue and look at its morphology - B- Provides enough thickness to account for cell to cell ratio
- A- Sample needs to be thin enough for light microscopy (about 1 cell layer thick)
12List method/s used for the collection of Cytology samples.
- A- Pap Smears, Endoscopy samples, some FNA (fine needle aspiration) specimens
- B- Blood test, saliva test, sputum collection
13Give an example of a Chemical Fixative that belongs to Aldehydes
- A- Formaldehyde, Glutaraldehyde
- B- Ethanol, Methanol
14Give an example of a Chemical Fixative that belongs to Oxidising agents
- A- Osmium tetroxide, Potassium Dichromate
- B- Ethanol, Methanol
15Give an example of a Chemical Fixative that belongs to Protein coagulants
- A- Ethanol, Methanol
- B- Mercuric Chloride, Picric acid
16Give an example of a Chemical Fixative that belongs to Uncertain mechanism
- A- Mercuric Chloride, Picric acid
- B- Ethanol, Methanol
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- Wk 8 - Foundations of Anatomical Pathology
- Author
- Jessica Bulley
- Language
- English
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- History
- Created on 5 May 2018 · updated on 5 May 2018
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