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Pigments in histopathology

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Quiz on Pigments in histopathology, created by Laura Currie on 10/28/2018.
Laura Currie
Quiz by Laura Currie, updated more than 1 year ago
Laura Currie
Created by Laura Currie almost 4 years ago
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Resource summary

Question 1

Question
What pigment is deposited in this picture?
Image:
Gh (binary/octet-stream)
Answer
  • Hemosiderin
  • Tattoo pigment
  • Lipofuschin
  • Debris

Question 2

Question
Von Kossa's method is for calcium
Answer
  • True
  • False

Question 3

Question
Hemosiderin is detected using [blank_start]Perls[blank_end] Reaction. Ferric iron deposits in tissue react with [blank_start]soluble[blank_end] ferrocyanide to form [blank_start]insoluble[blank_end] prussian blue dye
Answer
  • Perls
  • alizarin red
  • soluble
  • insoluble

Question 4

Question
Copper; (choose all correct options)
Answer
  • Stained with Perls
  • trace elements for enzyme reactions
  • High levels in liver may indicate alcoholic liver disease
  • Stained with alizarin red
  • Can be stained by Rhodanine stain

Question 5

Question
Anthracosis is also known as carbon lungs
Answer
  • True
  • False

Question 6

Question
Asbestosis is covered in iron containing protein coat; use Perls stain
Answer
  • True
  • False

Question 7

Question
Talc is biofringent
Answer
  • True
  • False

Question 8

Question
What colour is embolisation pigment?
Answer
  • Brown
  • Black
  • Yellow
  • Red

Question 9

Question
Tattoo pigments in skin tissue microscopy can be problematic with the diagnosis of [blank_start]melanoma[blank_end]
Answer
  • melanoma

Question 10

Question
Cholesterol [blank_start]clefts[blank_end] are due to [blank_start]xylene[blank_end] and alcohol dissolving cholesterol crystals.
Answer
  • clefts
  • domes
  • xylene
  • acid
  • Eosin

Question 11

Question
Melanin is an endogenous pigment
Answer
  • True
  • False

Question 12

Question
[blank_start]Bile[blank_end] pigments can be detected with Fouchet's bile stain.
Answer
  • Bile

Question 13

Question
What is Perls reaction positive for?
Answer
  • Hemosiderin
  • Hematoidin
  • Lipofuscin

Question 14

Question
What is Autofluorescence positive for?
Answer
  • Hemosiderin
  • Hematoidin
  • Lipofuscin

Question 15

Question
What is Schmorl's positive for?
Answer
  • Hemosiderin
  • Hematoidin
  • Lipofuscin

Question 16

Question
melanin is a tyrosine based pigment
Answer
  • True
  • False

Question 17

Question
The two types of melanin are [blank_start]dermal[blank_end] and [blank_start]neural[blank_end]
Answer
  • dermal
  • neural

Question 18

Question
[blank_start]Argentaffilic[blank_end] reaction binds silver and reduces it to black, whilst [blank_start]Argyrophilic[blank_end] binds silver but needs an external reducer such as formalin. (e.g. reticulin stain)
Answer
  • Argentaffilic
  • Argyrophilic
  • Argyrophilic
  • Argentaffilic

Question 19

Question
Melanin is Argentaffilic
Answer
  • True
  • False

Question 20

Question
Hydrogen peroxide bleach can be done to remove [blank_start]melanin[blank_end] pigment
Answer
  • melanin

Question 21

Question
Choose correct information in regards to Dermal melanin.
Answer
  • Polymerisation of tyrosine
  • Oxidation of dopamine
  • Schmorl's positive
  • H2O2 bleach takes 6+hours
  • Bleach takes less than 3 hours
  • DiPAS negative
  • DiPAS positive
  • Argentaffilic development time is 30 minutes
  • Argentaffilic development time is 60 minutes

Question 22

Question
Tick boxes below that are classified as aging pigments
Answer
  • Lipofuscin
  • Melanin
  • Russell bodies
  • Amyloid
  • Bile pigment
  • Corpora Amylecea
  • hemosiderin

Question 23

Question
Corpora Amylacea is PAS negative
Answer
  • True
  • False

Question 24

Question
Amyloid pigment is [blank_start]birefringent[blank_end] in polarised light after Congo red staining. It is an [blank_start]extracellular[blank_end] and proteinaceous. Several techniques should be used such as IHC and Crystal Violet for [blank_start]metachromasia[blank_end].
Answer
  • birefringent
  • invisible
  • metachromasia
  • polychromasia
  • extracellular
  • intracellular

Question 25

Question
What pigment is usually found in "Brown atrophy" of heart, adrenal glands and hepatic parenchymal cells? (They are gold/brown pigments)
Answer
  • Melanin
  • Lipofuscin
  • Hemosiderin
  • Hematoidin

Question 26

Question
What are lipofuscin positive for?
Answer
  • DiPAS
  • Luxol Fast blue
  • Schmorl's
  • Argyrophilic

Question 27

Question
Schmorle's reaction demonstrates oxidising substances
Answer
  • True
  • False

Question 28

Question
[blank_start]Melanin[blank_end] is Argentaffin and Schmorles positive, DiPAS negative and removed by H2O2 bleach. It is Long ZN negative, fat stain and Luxol Fast Blue negative. [blank_start]Lipofuscin[blank_end] on the other hand can be positive or negative Argentaffin and Schmorle's depending on timing, DiPAS positive, H2O2 bleach does not remove it, and positive for Long ZN, fat stains and Luxol fast blue.
Answer
  • Melanin
  • Lipofuscin
  • Hemosiderin
  • Bile
  • Lipofuscin
  • melanin
  • hemosiderin
  • bile

Question 29

Question
Melanosis is a condition of excessive melanin production in skin or other tissue
Answer
  • True
  • False

Question 30

Question
Formalin pigment is usually non-buffered formalin
Answer
  • True
  • False

Question 31

Question
Malaria pigment is birefringent
Answer
  • True
  • False

Question 32

Question
Mercury pigment is not birefringent
Answer
  • True
  • False
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