Quiz
Chapter 22- Papillomaviruses
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Questions in this quiz (116)
1Papillomaviruses infect eukaryotic cells.
- ATrue
- BFalse
2Human Papillomavirus Virus:
Envelope (Y or N): [blank_start]N[blank_end]
Capsid Shape: [blank_start]icosahedral[blank_end]
Diameter (in nm): [blank_start]55[blank_end] nm
T = [blank_start]7[blank_end] capsid
circular or linear genome: [blank_start]circular[blank_end]
DNA or RNA: [blank_start]DNA[blank_end]
ss or ds: [blank_start]ds[blank_end]
genome size: [blank_start]8[blank_end] kb
# of genes: [blank_start]8[blank_end] to [blank_start]10[blank_end] genes- AN
- Bicosahedral
- C55
- D7
- Ecircular
- FDNA
- Gds
- H8
- I8
- J10
3The genome of paillomavirus is packaged as a "[blank_start]mini[blank_end]chromosome" using cellular [blank_start]histones[blank_end].
- Amini
- Bhistones
4Paplliomaviruses use [blank_start]cDdDp[blank_end] as its polymerase for replication, and [blank_start]cDdRp[blank_end] as its polymerase for transcription.
- AcDdDp
- BcDdRp
5Replication of papillomavirus genome occurs in the [blank_start]nucleus[blank_end].
- Anucleus
6Papilloviruses only infect humans
- ATrue
- BFalse
7There are over [blank_start]100[blank_end] known types of papillomaviruses that infect humans
- A100
8Papillomaviruses infect humans, cows, dogs, rabbits etc.
- ATrue
- BFalse
9Papillomaviruses infect [blank_start]mucosa[blank_end] or [blank_start]skin[blank_end] cells
- Amucosa
- Bskin
10Transmission of papillomaviruses occur either through:
1.) [blank_start]skin contact[blank_end]
-OR-
2.) [blank_start]contaminated surfaces[blank_end]- Askin contact
- Bcontaminated surfaces
11Dieases from papillomaviruses include:
1.) [blank_start]benign warts[blank_end]
-OR-
2.) [blank_start]cancer[blank_end] (E.g. skin, cervical)- Abenign warts
- Bcancer
12Match the corresponding papillomavirus type with the site of infect and clinical manifestation:
Type 1- ASite of Infection: Soles of Feet
Clinical Manifestation: Deep Plantar Warts - BSite of Infection: Hands
Clinical Manifestation: Common Warts - CSite of Infection: Genital tract, larynx, nasal cavity
Clinical Manifestation: condyloma - DSite of Infection: Genital Tract
Clinical Manifestation: Cervical Cancer
- ASite of Infection: Soles of Feet
13Match the corresponding papillomavirus type with the site of infect and clinical manifestation:
Type 2, 4, 7- ASite of Infection: Soles of Feet
Clinical Manifestation: Deep Plantar Warts - BSite of Infection: Hands
Clinical Manifestation: Common Warts - CSite of Infection: Genital Tract, larynx, nasal cavity.
Clinical Manifestation: condyloma - DSite of Infection: Gential tract
Clinical Manifestation: cervical cancer
- ASite of Infection: Soles of Feet
14Match the corresponding papillomavirus type with the site of infect and clinical manifestation:
Type 6, 11- ASite of Infection: soles of feet
Clinical Manifestation: deep plantar warts - BSite of Infection: hands
Clinical Manifestation: common warts - CSite of Infection: genital tract, larynx, nasal cavity
Clinical Manifestation: condyloma - DSite of Infection: genital tract
Clinical Manifestation cervical cancer
- ASite of Infection: soles of feet
15Match the corresponding papillomavirus type with the site of infect and clinical manifestation:
Type 16, 18, 31- ASite of Infection: Soles of feet
Clinical Manifestation: deep plantar warts - BSite of Infection: hands
Clinical Manifestation: common warts - CSite of Infection: genital tract, larynx, nasal cavity
Clinical Manifestation: condyloma - DSite of Infection: genital tract
Clinical Manifestation: cervical cancer
- ASite of Infection: Soles of feet
16Types [blank_start]16[blank_end], [blank_start]18[blank_end] and [blank_start]31[blank_end] are main types for causing cervical cancer, these are targeted in vaccines.
- A16
- B18
- C31
17Papillomaviruses can cause both [blank_start]skin[blank_end] cancer and [blank_start]cervical[blank_end] cancer.
- Askin
- Bcervical
18condyloma means cervical [blank_start]warts[blank_end]. These are non cancerous but vaccines includes [blank_start]antigens[blank_end] against them.
- Awarts
- Bantigens
19Label the following UNINFECTED epithelium.
- Acorneal
- Bgranular
- Cspinous
- Dkeratinocytes
- Ebasal
20The basal layer is composed of [blank_start]undifferentiated[blank_end] stem cells. DNA synthesis and cell division [blank_start]does[blank_end] occur here
- Aundifferentiated
- Bdoes
21The spinous layer is composed of dividing keratinocytes
- ATrue
- BFalse
22The granular and corneal layer are composed of dead cells without any nucleus due to nuclear degradation.
- ATrue
- BFalse
23Keratinocytes are non-nucleated.
- ATrue
- BFalse
24Cells in the corneal and granular layers are non-nucleated.
- ATrue
- BFalse
25HPV viruses bind to [blank_start]heparin[blank_end] and [blank_start]alpha 6 integrin[blank_end], leading to endocytosis.
- Aheparin
- Balpha 6 integrin
26HPV replicates in the nucleus because its genome is made of [blank_start]DNA[blank_end].
- ADNA
27The initial site of infection of HPV occurs in the [blank_start]basal[blank_end] layer, infecting [blank_start]stem[blank_end] cells.
- Abasal
- Bstem
28[blank_start]Plasmid[blank_end] DNA replication occurs in the basal layer which is going to be [blank_start]bidirectional[blank_end] (directionality?).
[blank_start]Early[blank_end] genes are going to be expressed here.- APlasmid
- Bbidirectional
- CEarly
29[blank_start]50-100[blank_end] copies of the genome are made initially when HPV first infects.
- A50-100
30Every time the infected undifferentiated stem cell in the basal layer replicates, the initial infection of 50-100 viral particles are going to replicate along with it.
- ATrue
- BFalse
3150-100 viral particles are going to remain in the [blank_start]basal[blank_end] layer, whereas the other 50-100 viral particles are going to go into the [blank_start]spinous[blank_end] layer, where the [blank_start]keratinocytes[blank_end] are.
- Abasal
- Bspinous
- Ckeratinocytes
32Increased early gene expression of an infected epithelium is going to occur in the [blank_start]spinous[blank_end] layer, causing keratinocytes not to lose their [blank_start]nucleus[blank_end] and instead, begin replication
- Aspinous
- Bnucleus
33Veg. replication of viral genome becomes amplified due to [blank_start]rolling circle[blank_end] mechanism switch from bidirectional replication.
- Arolling circle
34veg. replication virl genome: [blank_start]bidirectional[blank_end] replication switches over to a high level of replication, rolling circle mechanism. amplification of viral DNA up to [blank_start]1000[blank_end] viral particles. At the same time, late gene expression occurs to synthesize [blank_start]capsid[blank_end] proteins which get imported into the [blank_start]nucleus[blank_end] and assembly occurs within the nucleus.
- Abidirectional
- B1000
- Ccapsid
- Dnucleus
35Once cell death occurs at the corneal and granular layer, the [blank_start]virions[blank_end] are released.
- Avirions
36[blank_start]6[blank_end] early replication proteins are made and [blank_start]2[blank_end] late capsid proteins are made.
- A6
- B2
37papillomavirus contains [blank_start]8[blank_end] genes in total
- A8
38Types [blank_start]16[blank_end] and [blank_start]31[blank_end] human papillomavirus are the types associated with cervical cancer
- A16
- B31
39Early gene expression of paillomaviruses is going to occur in the [blank_start]basal[blank_end] layer.
- Abasal
40E1 and E2 proteins are important proteins involved in genome replication.
- ATrue
- BFalse
41Which proteins are involved in cell proliferation?
- AE1
- BE2
- CE3
- DE4
- EE5
- FE6
- GE7
42Promotor late can either end at the:
1.) [blank_start]early termination signal[blank_end]
2.) [blank_start]late termination signal[blank_end]- Aearly termination signal
- Blate termination signal
43If promotor late ends at the early termination signal, it is going to produce [blank_start]E1[blank_end] and [blank_start]E2[blank_end] proteins, which are proteins important in replication, and makes them in large amounts via rolling circle mechanism.
- AE1
- BE2
44If promotor late ends at the late termination signal, it is going to make [blank_start]L1[blank_end] and [blank_start]L2[blank_end] proteins, which are the capsid proteins.
- AL1
- BL2
45If promotor late ends at the [blank_start]early[blank_end] termination signal, it is going to produce E1 and E2 proteins, which are proteins important in [blank_start]replication[blank_end], and makes them in large amounts via rolling circle mechanism, resulting in veg. replication.
- Aearly
- Breplication
46transcription of the papillomavirus occurs in one direction only, telling us that only one of the two strands is coding.
- ATrue
- BFalse
47When transcription occurs at promotor late, E6 and E7 proteins are synthesized
- ATrue
- BFalse
48Transcription from the early promotor is going to cause the expression of [blank_start]E5[blank_end], [blank_start]E6[blank_end], [blank_start]E7[blank_end], [blank_start]E1[blank_end] and [blank_start]E2[blank_end] proteins.
Where [blank_start]E5[blank_end], [blank_start]E6[blank_end], and [blank_start]E7[blank_end] are involved in cell proliferation and [blank_start]E1[blank_end] and [blank_start]E2[blank_end] are involved in plasmid replication.- AE5
- BE6
- CE7
- DE1
- EE2
- FE5
- GE6
- HE7
- IE1
- JE2
49Proteins E1 and E2 are involved in cell proliferation
- ATrue
- BFalse
50Proteins E1 and E2 are involved in plasmid replication
- ATrue
- BFalse
51Proteins E5, E6, and E7 are involved in plasmid replication
- ATrue
- BFalse
52Proteins E5, E6, and E7 are involved in cell proliferation.
- ATrue
- BFalse
53To get expression of L1 and L2, you have to splice out [blank_start]E1[blank_end] and [blank_start]E4[blank_end] coding regions so that L2 is now the opening reading frame of the first message.
- AE4
- BE1
54E5 is part of L2 structural protein
- ATrue
- BFalse
55if you want to express E7 you have to splice out E6 so that E7 is going to be the first one expressed now.
- ATrue
- BFalse
56Match the correct protein with the following function:
DNA helicase, binds to replication origin, initiates DNA replication.- AE1
- BE2
- CE3
- DE4
- EE5
- FE6
- GE7
- HL1
- IL2
57Match the correct protein with the following function:
Binds to specific sites on viral DNA, enhancers binding of E1 to replication origin, activates or represses transcription, binds to mitotic chromosomes and aids in seperation of viral genomes.- AE1
- BE2
- CE3
- DE4
- EE5
- FE6
- GE7
58E1 and E2 proteins both bind ori.
- ATrue
- BFalse
59[blank_start]E2[blank_end] helps E1 bind to ori. In addition, this protein can activate or repress viral protein expression (early +late).
- AE2
60E2 can help activate or repress viral protein expression, early or late.
- ATrue
- BFalse
61E1 can activate or repress viral protein expression (early or late).
- ATrue
- BFalse
62E3 is present in all palliomaviruses.
- ATrue
- BFalse
63The function of _______ is unknown.
- AE1
- BE2
- CE4
- DE5
- EE6
- FE7
- GL1
- HL2
64Select the protein with the corresponding function.
Enhances growth factor repsonses in infected cell, stimulates cell proliferation- AE1
- BE2
- CE4
- DE5
- EE6
- FE7
65Directs ubiquitin-mediated degradation of cellular p53 and other target proteins, inhibits cell-cycle block and apoptosis (in oncogenic papillomaviruses only)
- AE1
- BE2
- CE4
- DE5
- EE6
- FE7
66Binds and degrades Rb protein, releases E2F, activates cell cycling and DNA replication genes.
- AE1
- BE2
- CE4
- DE5
- EE6
- FE7
67Every papillomavirus contains E8 protein.
- ATrue
- BFalse
68E5 -> stimulates cell [blank_start]proliferation[blank_end]
E6 -> inhibits cell cycle [blank_start]block[blank_end]
E7 -> [blank_start]activates[blank_end] cell cycle and DNA replication genes to activate [blank_start]keratinocyte[blank_end] replication- Aproliferation
- Bblock
- Cactivates
- Dkeratinocyte
69DNA genome replication by [blank_start]cDdDp[blank_end] in differentiated keratinocytes occur.
- AcDdDp
70Oncogenes are genes that repress cancer.
- ATrue
- BFalse
71HPV E7 and HPV E6 are the only tumor virus oncogenes known to date.
- ATrue
- BFalse
72Adenoviruses can also have oncogenes.
- ATrue
- BFalse
73SV40V also possess oncogenes.
- ATrue
- BFalse
74Examples of cell tumor suppressor genes include:
1. [blank_start]pRB[blank_end]
2. [blank_start]p53[blank_end]- ApRB
- Bp53
75[blank_start]p53[blank_end] is mutated in >50% of human tumors.
- Ap53
76pRB is an example of a gene that contributes to the repression of cancer.
- ATrue
- BFalse
77[blank_start]E7[blank_end] binds to Rb and inactivates it, causing cancer.
- AE7
78[blank_start]E6[blank_end] binds to p53 and inactivates it.
- AE6
79When E2F is free, it is [blank_start]active[blank_end].
- Aactive
80[blank_start]Rb[blank_end] binds to E2F to prevent it from activating the cell cycle.
- ARb
81Rb, when phosphorylated by CDK, it unable to bind to [blank_start]E2F[blank_end] and DNA replication occurs.
- AE2F
82There are two ways to turn Rb off:
1.) [blank_start]CDK[blank_end]
2.) [blank_start]E7[blank_end]- ACDK
- BE7
83When Rb is phosphorylated, [blank_start]E2F[blank_end] will be free and active.
- AE2F
84E7 binding to Rb sterically prevents it from binding to E2F, allowing for cell cycle progression.
- ATrue
- BFalse
85In order for E7 for be oncogenic, it needs to bind [blank_start]tightly[blank_end] to Rb.
- Atightly
86As long as E7 binds to Rb, it will be oncogenic + cause cancer.
- ATrue
- BFalse
87E7 can bind to Rb, sterically blocking its interaction with E2F and can also direct Rb to be [blank_start]ubiquitinated[blank_end].
- Aubiquitinated
88Once the cell cycle is induced by E7, replication machinery such as [blank_start]cDdDp[blank_end] is induced which is going to be used by the virus.
- AcDdDp
89[blank_start]p21[blank_end] inhibits CDK.
- Ap21
90[blank_start]p53[blank_end] senses unscheduled DNA replication and activates a protein called p21 which in turn inhibits CDK.
- Ap53
91p53 causes the phosphorylation of Rb.
- ATrue
- BFalse
92E2F is a [blank_start]transcription[blank_end] factor.
- Atranscription
93Rb, when phosphorylated by CDK, can not bind to [blank_start]E2F[blank_end], activating cellular DNA synthesis.
- AE2F
94Free E2F activates transcription of cell-cycle genes, making [blank_start]cDdDp[blank_end] for the virus.
- AcDdDp
95p53 can cause cell cycle arrest by activating [blank_start]p21[blank_end] to block CDK .
- Ap21
96E7 activates p53
- ATrue
- BFalse
97DNA damage activates p53.
- ATrue
- BFalse
98p53 activates transcription of [blank_start]p21[blank_end] and [blank_start]Bax[blank_end] protein.
- Ap21
- BBax
99Bax proteins, activated by [blank_start]p53[blank_end], induces [blank_start]apoptosis[blank_end].
- Aapoptosis
- Bp53
100E6 is able to get rid of p53.
- ATrue
- BFalse
101E6 binds to cellular [blank_start]ubiquitin ligase[blank_end], which binds to p53 and causes degradation in proteasomes.
- Aubiquitin ligase
102E6 can mediate p53 degradation
- ATrue
- BFalse
103E6 binds to Rb to induce cancer
- ATrue
- BFalse
104Only E7 from oncogenic types mediate p53 degradation
- ATrue
- BFalse
105p53 is not mutated in cervical cancers caused by [blank_start]HPV[blank_end]
- AHPV
106[blank_start]E6[blank_end] and [blank_start]E7[blank_end] become integrated into a cellular genome, where expression results in "transformation" into a cancer cell.
- AE6
- BE7
107Viruses can replicate in transformed cancer cells (HeLa cell line)
- ATrue
- BFalse
108HPV cancers are the most prevalent in [blank_start]developing[blank_end] countries
- Adeveloping
109p53 is involved in all cervical tumors.
- ATrue
- BFalse
110VLP stands for [blank_start]virus like particles[blank_end].
- Avirus like particles
111Virus like particles are like coat proteins without a genome inside
- ATrue
- BFalse
112L1 from oncogenic types is used in virus like particle vaccinations
- ATrue
- BFalse
113Gardasil vaccine, [blank_start]June[blank_end] 2006, works for virus types [blank_start]6[blank_end]. [blank_start]11[blank_end], [blank_start]16[blank_end] and [blank_start]18[blank_end]
- AJune
- B6
- C11
- D16
- E18
114[blank_start]Cervarix[blank_end] vaccine has type 16 and 18 L1s as its VLPs.
- ACervarix
115[blank_start]Gardasil[blank_end] vaccine works with types 16, 18 and 6 and 11.
- AGardasil
116the long control region is the site of transcriptional [blank_start]regulation[blank_end] and is the [blank_start]ori[blank_end] site
- Aregulation
- Bori
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- Description
- Quiz on Chapter 22- Papillomaviruses, created by Nina Butris on 17/02/2019.
- Author
- Nina Butris
- Language
- English
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