Quiz
Chapter 14, 12 & 7
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Questions in this quiz (23)
1Label correctly
- ARestriction enzymes make staggered cuts
- BProducing single-stranded sticky ends
- Cother restriction enzymes (Pvull)
- Dcut straight across making blunt ends
2Steps in Recombinant DNA technology:
1. [blank_start]Isolate[blank_end] the DNA segment or gene from remaining DNA
2. Use [blank_start]restriction enzymes[blank_end] to cut and join DNA fragments from 2 different sources
3. [blank_start]View[blank_end] DNA fragments
4. Locate DNA fragments with [blank_start]Southern Blotting and Probes[blank_end].- AIsolate
- Brestriction enzymes
- CView
- DSouthern Blotting and Probes
3Which is the restriction enzyme that makes staggered cuts in DNA producing sticky ends?
- AHind III
- BPvu II
- CHis -
4Which enzyme makes straight cuts and produces blunt ends during DNA recombination?
- AHind III
- BPvu II
- CHis -
5Examples of Recombinant DNA (rDNA)
[blank_start]insulin[blank_end]
[blank_start]Human growth hormone[blank_end]
[blank_start]some vaccines[blank_end]
[blank_start]crops with GMO's[blank_end]- Ainsulin
- Bestrogen
- CHuman growth hormone
- Dtestosterone
- Esome vaccines
- Fpolio
- Gcrops with GMO's
- Hcorn
6What is the purpose of the PCR (Polymerase-Chain reaction)?
- Ainduce DNA replication in a test tube
- Bto target a specific gene
- Cadd in DNA recombinant technology
7Label correctly
- ADNA is heated to separate strands
- BDNA quickly cooled, primers anneal
- CHeated, DNA synthesis occurs
- DCycle is repeated
8Which enzyme is used during PCR
- ATaq polymerase
- BLigase
- CPrimase
9A [blank_start]southern blot[blank_end] confirms the DNA from a gel is the target DNA.
A [blank_start]probe[blank_end] is DNA/RNA with a base sequence complementary to a sequence in the gene of interest.
If probes combine at correct spots that confirms the correct [blank_start]target gene[blank_end].- Asouthern blot
- Bprobe
- Ctarget gene
10[blank_start]Gene cloning[blank_end] amplifies a specific piece of DNA via a bacteria cell.
[blank_start]Vectors[blank_end] determine what is going to transport DNA into a cell.
[blank_start]Transformation[blank_end] allows bacteria to take in foreign DNA.
[blank_start]Restriction site[blank_end] is the spot to cut plasmid.
[blank_start]Ori[blank_end] is the origin of replication.
[blank_start]Selectable markers[blank_end] screen for prescence of DNA.- AGene cloning
- BVectors
- CTransformation
- DRestriction site
- EOri
- FSelectable markers
11A library consisting only of those DNA sequences that are transcribed into mRNA
- AcDNA library
- BmRNA library
- Ctranscript library
12Label correctly
- ADNA digested by restriction enzymes
- BDNA cut in different places
- Cfragments joined to cloning vectors
- D& transferred to a bacterial cell
- Eclones contain overlapping fragments
13Screening a DNA library
- Amembrane laid on top of bacteria colony
- Bfew bacteria cells adhere to membrane
- CDNA denatured & fixed to filter
- Dprobe hybridizes w/ complimentary DNA
- Eexcess probe removed, X-ray film applied
- FFilm detects presence of probe
- Gcomparison shows DNA of interest
14Sanger's Deoxy Sequencing Method
- Asingle-stranded DNA fragment is isolated
- BEach ddNTP tagged with different dye
- CSanger Sequencing Reaction carried out
- Dfragment with same end base have same dy
- EDNA denatured, loaded into gel & migrate
- FFluorescent dye detected by a laser beam
- Gfragments appear as colored peaks
- Hsequence read into computer
15What organisms are unicellular, lack a membrane bound nucleus and membrane bound organelles?
- AProkaryotes
- BEukaryotes
- CHumanoids
16[blank_start]Prototrophic[blank_end]: wild type
[blank_start]Auxotropic[blank_end]: mutant type
[blank_start]Minimum media[blank_end]: only required by prototrophic bacteria
[blank_start]Complete medium[blank_end]: contain all substances required by all bacteria, including auxotrophic- APrototrophic
- BAuxotropic
- CMinimum media
- DComplete medium
17Extra small, circular chromosomal DNA is known as
- Aplasmids
- Borganelles
- Cmitochondria
18Gene transfer in bacteria:
[blank_start]Conjugation[blank_end]: direct transfer of DNA from on bacterium to another
[blank_start]Transformation[blank_end]: bacterium takes up free DNA
[blank_start]Transduction[blank_end]: bacterial viruses take DNA from one bacterium to another- AConjugation
- BTransformation
- CTransduction
19[blank_start]Leaderberg and Tatum[blank_end] did a Conjugation experiment:
[blank_start]F+ cells[blank_end]: donor cells with the F factor
[blank_start]F- cells[blank_end]: recipient cells lacking F factor
[blank_start]Sex pilus[blank_end]: connection tube- ALeaderberg and Tatum
- BF+ cells
- CF- cells
- DSex pilus
20bacterium with a conjugative plasmid (for example, the F-factor) integrated into its chromosomal DNA. The integration of the plasmid into the cell's chromosome is through homologous recombination.
- AA high-frequency recombination cell (Hfr cell)
- BPvu II
- CHind III
21Antibiotic Resistance
[blank_start]Antibiotic resistance[blank_end] comes from the actions of genes located on [blank_start]R plasmids[blank_end] that can be transferred naturally
R plasmids have evolved in the past 60 years since the beginning of widespread use of [blank_start]antibiotics[blank_end].
The transfer of R plasmids is not restricted to bacteria of the [blank_start]same or even related[blank_end] species.- AAntibiotic resistance
- BR plasmids
- Cantibiotics
- Dsame or even related
22A bacterial virus (bacteriophage) carries DNA from one bacterium to another and usually occurs within same or closely related species.
- Atransduction
- Btranscription
- Ctranslocation
23Gene Expression:
[blank_start]Constitutive expression[blank_end]: continuously expressed under normal cellular conditions and not regulated
[blank_start]Positive Control[blank_end]: stimulate gene expression
[blank_start]Negative control[blank_end]: inhibits gene expression
[blank_start]Operon[blank_end]: transcriptional unit that contains functionally related genes that include the [blank_start]Promoter[blank_end], [blank_start]Operator[blank_end], and [blank_start]structure genes[blank_end]
[blank_start]Regulator gene[blank_end]: not part of the operon, DNA sequence encoding products that affect the operon function, helps control expression of the structural genes of the operon- AConstitutive expression
- BPositive Control
- CNegative control
- DOperon
- ERegulator gene
- FPromoter
- GOperator
- Hstructure genes
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- Description
- Quiz on chapters 12 and 14 from Genetics Essentials, 4th Edition
- Author
- Raegan Ferro-Gaskin
- Language
- English
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