Introduction & Physiology of Industrial and Environmental Microbiology

Matthew Coulson
Quiz by Matthew Coulson, updated 11 months ago
Matthew Coulson
Created by Matthew Coulson 11 months ago
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Description

The first afternoon of the course - first lecture given.

Resource summary

Question 1

Question
Alcoholic beverages typically utilise yeasts in their production, especially [blank_start]Saccharomyces cerevisiae[blank_end] (commonly known as baker's yeast).
Answer
  • Saccharomyces cerevisiae
  • Saccharomyces boulardii
  • Saccharomyces eubayanus
  • Saccharomyces carlsbergensis

Question 2

Question
Vinegar is scientifically known as [blank_start]acetic[blank_end] acid
Answer
  • acetic

Question 3

Question
Which of the following fungi is used in the production of citric acid?
Answer
  • Aspergillus Niger
  • Aspergillus Nidulans
  • Aspergillus Oryzae
  • Aspergillus Fumigatus
  • Aspergillus Flavus

Question 4

Question
Antibiotics are [blank_start]secondary[blank_end] metabolites synthesized by filamentous bacteria and fungi
Answer
  • secondary
  • primary
  • tertiary

Question 5

Question
Which type of enzyme is found in washing powders?
Answer
  • Proteases
  • Amylases
  • Lipases
  • Cellulase

Question 6

Question
Which two enzymes are the main regulators of glucose breakdown?
Answer
  • Alpha amylase
  • Beta amylase
  • Delta amylase
  • Amyloglucosidase
  • Glucoamylase

Question 7

Question
[blank_start]Prokaryotes[blank_end] are organisms made up of cells that lack a cell nucleus or any membrane-encased organelles. [blank_start]Eukaryotes[blank_end] are organisms made up of cells that possess a membrane-bound nucleus that holds genetic material as well as membrane-bound organelles.
Answer
  • Eukaryotes
  • Prokaryotes

Question 8

Question
Prokaryote or Eukaryote? Archaea = [blank_start]Prokaryote[blank_end] Fungi = [blank_start]Eukaryote[blank_end] Protozoa = [blank_start]Eukaryote[blank_end] Algae = [blank_start]Eukaryote[blank_end] Bacteria = [blank_start]Prokaryote[blank_end]
Answer
  • Prokaryote
  • Eukaryote
  • Prokaryote
  • Eukaryote
  • Eukaryote
  • Prokaryote
  • Eukaryote
  • Prokaryote
  • Eukaryote
  • Prokaryote

Question 9

Question
There are 3 types of Archaea: [blank_start]Halophiles[blank_end] - Adapted to environments with high salt concentrations [blank_start]Methanogens[blank_end] - Archaea which produce methane [blank_start]Thermophiles[blank_end] - Adapted to environments with extremely high temperatures
Answer
  • Halophiles
  • Methanogens
  • Thermophiles

Question 10

Question
Thermus Aquaticus gives rise to which useful genetic enzyme? [blank_start]Taq Polymerase[blank_end]
Answer
  • Taq Polymerase

Question 11

Question
Almost all industrial bacteria are members of which two subgroups of Eubacteria?
Answer
  • Proteobacteria
  • Gram Positive Eubacteria
  • Chlamydia
  • Cyanobacteria
  • Spirochetes

Question 12

Question
One of the defining features of prokaryotic organisms is membrane bound organelles
Answer
  • True
  • False

Question 13

Question
The genetic material of Prokaryotes is typically found within [blank_start]a single circular chromosome of DNA[blank_end]
Answer
  • a single circular chromosome of DNA
  • multiple parallel chromosomes
  • a number of interconnected plasmids

Question 14

Question
Prokaryotes conduct cell division via [blank_start]binary fission[blank_end] Eukaryotes conduct cell division via [blank_start]mitosis[blank_end]
Answer
  • mitosis
  • binary fission
  • binary fission
  • mitosis

Question 15

Question
Gram [blank_start]positive[blank_end] organisms have 1 cell membrane and a thick layer of peptidoglycan Gram [blank_start]negative[blank_end] organisms have 2 cell membranes and a thin layer of peptidoglycan in between
Answer
  • negative
  • positive
  • positive
  • negative

Question 16

Question
The inner membrane of a gram negative organism is also known as the [blank_start]cytoplasmic[blank_end] membrane
Answer
  • cytoplasmic

Question 17

Question
[blank_start]Escherichia coli[blank_end] is a gram negative bacterium which is a facultative anaerobe commonly used in the production of heterologous industrial microorganisms [blank_start]Bacillus Subtilis[blank_end] is a gram positive bacterium which is a facultative anaerobe commonly used in the production of industrial enzymes such as amylases and proteases
Answer
  • Bacillus Subtilis
  • Escherichia Coli
  • Escherichia Coli
  • Bacillus Subtilis

Question 18

Question
Eukaryotes are typically [blank_start]larger[blank_end] than prokaryotes
Answer
  • larger
  • smaller

Question 19

Question
In eukaryotes, the nucleus is usually bound within a [blank_start]double membrane[blank_end]
Answer
  • double membrane
  • single membrane

Question 20

Question
The cell walls of eukaryotes consist mainly of [blank_start]cellulose[blank_end] The cell walls of fungi consist mainly of [blank_start]chitin[blank_end]
Answer
  • chitin
  • cellulose

Question 21

Question
What are the two broad subgroups of Fungi? (alphabetical order, plural) [blank_start]Molds[blank_end] [blank_start]Yeasts[blank_end]
Answer
  • Molds
  • Yeasts

Question 22

Question
Match the subtype of Fungi to its structure: Mold - [blank_start]Filamentous Hyphae[blank_end] Yeast - [blank_start]Unicellular[blank_end]
Answer
  • Filamentous Hyphae
  • Unicellular

Question 23

Question
Which of the four main classes of Fungi contain the Yeasts?
Answer
  • Phycomycetes
  • Ascomycetes
  • Basidomycetes
  • Deuteromycetes

Question 24

Question
Microorganisms can be classified into two broad categories based on their energy source: [blank_start]Chemotrophs[blank_end] = Obtain energy by oxidation of organic or inorganic molecules [blank_start]Phototrophs[blank_end] = Obtains energy via light Microorganisms can also be classified into two groups based on their carbon source: [blank_start]Heterotrophs[blank_end] = Utilise a wide range of organic molecules as their carbon source [blank_start]Autotrophs[blank_end] = Utilise CO2 as their sole or primary carbon source
Answer
  • Autotrophs
  • Heterotrophs
  • Phototrophs
  • Chemotrophs

Question 25

Question
Most fermentation reactions are [blank_start]heterotrophic[blank_end]
Answer
  • heterotrophic
  • autotrophic

Question 26

Question
[blank_start]Prototrophs[blank_end] are microorganisms that can grow on minimal media containing only carbon and energy sources and basic mineral elements [blank_start]Auxotrophs[blank_end] are microorganisms that require additional organic substances such as amino acids or vitamins
Answer
  • Prototrophs
  • Auxotrophs

Question 27

Question
Microbial Growth Kinetics: [blank_start]Batch growth[blank_end] = Closed system whereby all nutrients are present at the start of the process. [blank_start]Fed-batch growth[blank_end] = Fresh medium is added as the reaction progresses but no old medium is removed. [blank_start]Continuous growth[blank_end] = Fresh medium is continuously fed into the fermentation vessel but used medium is removed at the same rate, creating optimum conditions for growth.
Answer
  • Continuous growth
  • Fed-batch growth
  • Batch growth
  • Intermittent growth

Question 28

Question
The main difference between Fed-batch and Continuous growth is that: In [blank_start]Fed batch growth[blank_end] the overall volume increases as the reaction progresses In [blank_start]Continuous growth[blank_end] the overall volume remains constant throughout the reaction
Answer
  • Continuous growth
  • Fed batch growth
  • Fed batch growth
  • Continuous growth

Question 29

Question
What are the four growth phases of a microorganism? [blank_start]Lag[blank_end] Phase: This initial phase is characterized by cellular activity but not growth. Cells are placed in media which allows them to synthesize proteins and other molecules necessary for replication however they do not actually divide. [blank_start]Exponential[blank_end] Phase: This is the time when the cells are dividing by binary fission and doubling in numbers after each generation time. [blank_start]Stationary[blank_end] Phase: Available nutrients become depleted and waste products start to accumulate. Bacterial cell growth reaches a plateau, or stationary phase, where the number of dividing cells equal the number of dying cells. [blank_start]Death[blank_end] Phase: As nutrients become less available and waste products increase, the number of dying cells continues to rise. The number of living cells decreases exponentially and population growth experiences a sharp decline.
Answer
  • Death
  • Stationary
  • Exponential
  • Lag

Question 30

Question
In which growth phase are antibiotics typically most effective? (See description for details)
Answer
  • Lag
  • Exponential (Log)
  • Stationary
  • Death

Question 31

Question
Primary metabolism produces products essential for growth and replication. This comprises the [blank_start]Lag and Exponential[blank_end] Phases and is also known as [blank_start]Trophophase[blank_end]. Secondary metabolism produces products important in ecological and other activities of the cell. This comprises the [blank_start]Stationary and Death[blank_end] Phases and is also known as [blank_start]Idiophase[blank_end].
Answer
  • Trophophase
  • Idiophase
  • Stationary and Death
  • Lag and Exponential

Question 32

Question
Antibiotics are produced by the [blank_start]secondary metabolism[blank_end]
Answer
  • secondary metabolism
  • primary metabolism

Question 33

Question
The 'Disadvantages of Batch Fermentation' slide (slide 37) seems very examinable. This could easily come up as 'Compare and contrast the different methods of fermentation' e.g. comparing batch and fed-batch to continuous. Go over and remember a few of the differences, such as: - Batch fermentation means that the system never achieves a steady state due to the constantly changing conditions of the environment. - Batch fermentation involves a lot of practical steps and procedures which gives more room for human error and contamination - Theoretically, the exponential phase in a continuous culture can be extended indefinitely as if you keep providing new material and removing old, used culture material the organism will never run out of nutrients. Click 'True' to continue.
Answer
  • True
  • False
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