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Microbiology Lab Practical Staining

Meagan Mueller 7 questions Updated 1 Nov 2016

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Questions in this quiz (7)

  1. 1[blank_start]Simple[blank_end] staining uses [blank_start]basic[blank_end] [blank_start]dyes[blank_end] such as crystal violet, [blank_start]methylene[blank_end] blue, safrinin, [blank_start]malachite[blank_end] green, and basic fuchsin. These stains can show us if the cell is a [blank_start]prokaryote[blank_end] or [blank_start]eukaryote[blank_end]. They also show morphology such as [blank_start]shape[blank_end] and [blank_start]arrangement[blank_end].
    1. ASimple
    2. Bbasic
    3. Cdyes
    4. Dmethylene
    5. Emalachite
    6. Fprokaryote
    7. Geukaryote
    8. Hshape
    9. Iarrangement
  2. 2[blank_start]Differential[blank_end] staining uses two or more stains. Examples include the [blank_start]Gram[blank_end] stain and [blank_start]acid-fast[blank_end] stain. The latter is used for cells that have [blank_start]mycolic[blank_end] [blank_start]acid[blank_end] in their cell wall.
    1. ADifferential
    2. BGram
    3. Cacid-fast
    4. Dmycolic
    5. Eacid
  3. 3Step 1: [blank_start]Primary[blank_end] stain = crystal violet. All cells are [blank_start]purple[blank_end]. Step 2: Mordant = [blank_start]iodine[blank_end]. All cells are [blank_start]purple[blank_end]. Step 3: Decolorizer = [blank_start]alcohol[blank_end]. Gram negative cells are [blank_start]colorless[blank_end]. Gram positive cells are [blank_start]purple[blank_end]. Step 4: counterstain = [blank_start]safrinin[blank_end]. Gram negative cells are [blank_start]pink[blank_end]. Gram positive cells are [blank_start]purple[blank_end]. Acid fast cells are [blank_start]colorless[blank_end].
    1. APrimary
    2. Bpurple
    3. Ciodine
    4. Dpurple
    5. Ealcohol
    6. Fcolorless
    7. Gpurple
    8. Hsafrinin
    9. Ipink
    10. Jpurple
    11. Kcolorless
  4. 4The [blank_start]decolorizer[blank_end] creates holes in the [blank_start]lipopolysaccharides[blank_end] of Gram [blank_start]negative[blank_end] cell wall and washes out the [blank_start]iodine[blank_end] and [blank_start]crystal[blank_end] [blank_start]violet[blank_end]. The thick layer of [blank_start]peptidoglycan[blank_end] in Gram [blank_start]positive[blank_end] cell wall retains [blank_start]crystal[blank_end] [blank_start]violet[blank_end].
    1. Adecolorizer
    2. Blipopolysaccharides
    3. Cnegative
    4. Diodine
    5. Ecrystal
    6. Fviolet
    7. Gpeptidoglycan
    8. Hpositive
    9. Icrystal
    10. Jviolet
  5. 5Label:Image for question 5
    1. Apeptidoglycan
    2. Bteichoic acid
    3. Clipoteichoic acid
    4. Dgram positive
  6. 6Label:Image for question 6
    1. Alipopolysaccharides
    2. Bporin protein
    3. Cphospholipids
    4. Dpeptidoglycan
    5. EGram negative
  7. 7Label:Image for question 7
    1. Aprimary dye
    2. Bmordant
    3. Cdecolorizer
    4. Dcounterstain

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Description
Quiz on Microbiology Lab Practical Staining, created by Meagan Mueller on 31/10/2016.
Language
English
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Created on 31 Oct 2016 · updated on 1 Nov 2016
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