Microbiology Lab Practical Staining

1. 1[blank_start]Simple[blank_end] staining uses [blank_start]basic[blank_end] [blank_start]dyes[blank_end] such as crystal violet, [blank_start]methylene[blank_end] blue, safrinin, [blank_start]malachite[blank_end] green, and basic fuchsin. These stains can show us if the cell is a [blank_start]prokaryote[blank_end] or [blank_start]eukaryote[blank_end]. They also show morphology such as [blank_start]shape[blank_end] and [blank_start]arrangement[blank_end].

   1. ASimple
   2. Bbasic
   3. Cdyes
   4. Dmethylene
   5. Emalachite
   6. Fprokaryote
   7. Geukaryote
   8. Hshape
   9. Iarrangement
2. 2[blank_start]Differential[blank_end] staining uses two or more stains. Examples include the [blank_start]Gram[blank_end] stain and [blank_start]acid-fast[blank_end] stain. The latter is used for cells that have [blank_start]mycolic[blank_end] [blank_start]acid[blank_end] in their cell wall.

   1. ADifferential
   2. BGram
   3. Cacid-fast
   4. Dmycolic
   5. Eacid
3. 3Step 1: [blank_start]Primary[blank_end] stain = crystal violet. All cells are [blank_start]purple[blank_end]. Step 2: Mordant = [blank_start]iodine[blank_end]. All cells are [blank_start]purple[blank_end]. Step 3: Decolorizer = [blank_start]alcohol[blank_end]. Gram negative cells are [blank_start]colorless[blank_end]. Gram positive cells are [blank_start]purple[blank_end]. Step 4: counterstain = [blank_start]safrinin[blank_end]. Gram negative cells are [blank_start]pink[blank_end]. Gram positive cells are [blank_start]purple[blank_end]. Acid fast cells are [blank_start]colorless[blank_end].

   1. APrimary
   2. Bpurple
   3. Ciodine
   4. Dpurple
   5. Ealcohol
   6. Fcolorless
   7. Gpurple
   8. Hsafrinin
   9. Ipink
   10. Jpurple
   11. Kcolorless
4. 4The [blank_start]decolorizer[blank_end] creates holes in the [blank_start]lipopolysaccharides[blank_end] of Gram [blank_start]negative[blank_end] cell wall and washes out the [blank_start]iodine[blank_end] and [blank_start]crystal[blank_end] [blank_start]violet[blank_end]. The thick layer of [blank_start]peptidoglycan[blank_end] in Gram [blank_start]positive[blank_end] cell wall retains [blank_start]crystal[blank_end] [blank_start]violet[blank_end].

   1. Adecolorizer
   2. Blipopolysaccharides
   3. Cnegative
   4. Diodine
   5. Ecrystal
   6. Fviolet
   7. Gpeptidoglycan
   8. Hpositive
   9. Icrystal
   10. Jviolet
5. 5Label:

   

   1. Apeptidoglycan
   2. Bteichoic acid
   3. Clipoteichoic acid
   4. Dgram positive
6. 6Label:

   

   1. Alipopolysaccharides
   2. Bporin protein
   3. Cphospholipids
   4. Dpeptidoglycan
   5. EGram negative
7. 7Label:

   

   1. Aprimary dye
   2. Bmordant
   3. Cdecolorizer
   4. Dcounterstain

Description

Quiz on Microbiology Lab Practical Staining, created by Meagan Mueller on 31/10/2016.

Author

Meagan Mueller

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Created on 31 Oct 2016 · updated on 1 Nov 2016

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