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Specialized media
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1[blank_start]General[blank_end] [blank_start]purpose[blank_end] media is used to grow bacteria and fungi that are not fastidious. It is nonselective, complex formula with milk protein, soybean protein and sodium chloride. examples: [blank_start]tryptic[blank_end] [blank_start]soy[blank_end], nutrient agar, and [blank_start]brain[blank_end] [blank_start]heart[blank_end] [blank_start]infusion[blank_end]
- AGeneral
- Bpurpose
- Ctryptic
- Dsoy
- Ebrain
- Fheart
- Ginfusion
2Thioglycolate broth is a [blank_start]reducing[blank_end] media that can determine [blank_start]oxygen requirements[blank_end] and allows all organisms to grow including [blank_start]anaerobic[blank_end] organisms
- Areducing
- Boxygen requirements
- Canaerobic
3[blank_start]Transport[blank_end] media is used to keep bacteria alive until it can be cultured in a lab. It has buffers and [blank_start]salts[blank_end] that [blank_start]inhibit[blank_end] growth. Ex: Annie's Stuarts and Cary Blair
- ATransport
- Bsalts
- Cinhibit
4[blank_start]Enriched[blank_end] media has added nutrient from blood, serum, hemoglobin, eggs, or growth factors such as vitamins and nutrients. It allows [blank_start]fastidious[blank_end] organisms to grow. Examples: blood agar and [blank_start]chocolate[blank_end] agar
- AEnriched
- Bfastidious
- Cchocolate
5[blank_start]Selective[blank_end] media inhibits one group of organisms while allowing another to grow. It has chemical additives such as salts, bile salts, dyes, alcohols, and antibiotics. It can be used to [blank_start]inhibit[blank_end] normal flora to identify [blank_start]pathogens[blank_end]
- ASelective
- Binhibit
- Cpathogens
6[blank_start]Differential[blank_end] media shows when a particular biochemical reaction has taken place to identify an organism. If the organism as a certain bacterial [blank_start]enzyme[blank_end] and biochemical reaction takes place and the color changes.
- ADifferential
- Benzyme
7Phenylethyl agar is [blank_start]selective[blank_end] for gram [blank_start]positive[blank_end] organisms (s. aureus) and [blank_start]bacteriostatic[blank_end] for gram [blank_start]negative[blank_end] organisms (e. coli). It dissolves [blank_start]lipopolysaccharides[blank_end] in cell wall and inhibits DNA synthesis
- Aselective
- Bpositive
- Cbacteriostatic
- Dnegative
- Elipopolysaccharides
8Thioglycolate broth is [blank_start]enriched[blank_end] and [blank_start]differential[blank_end]. It allows all organisms to grow including anaerobic.
- Aenriched
- Bdifferential
9MacConkey's agar: [blank_start]selective[blank_end] because it inhibits gram [blank_start]positive[blank_end] organisms and allows gram [blank_start]negative[blank_end] enterobacteriacea to grow. also [blank_start]differential[blank_end] because lactose fermentation which leads to acid production shows if it is e. coli ([blank_start]bright[blank_end] [blank_start]pink[blank_end] when fermentation causes acid production)
- Aselective
- Bpositive
- Cnegative
- Ddifferential
- Ebright
- Fpink
10Eosin methylene blue agar: [blank_start]differential[blank_end] when the fermentation of lactose leads to acid production (lactose positive = E. coli) and no acid production (lactose neg = salmonella/shigella) also [blank_start]selective[blank_end] allows gram [blank_start]negative[blank_end] but inhibits gram [blank_start]positive[blank_end]
- Adifferential
- Bselective
- Cnegative
- Dpositive
11Blood agar is [blank_start]enriched[blank_end] and [blank_start]differential[blank_end]. It consists of tryptic soy and [blank_start]5%[blank_end] sheep or rabbit blood. It grows [blank_start]fastidious[blank_end] organisms like staphylococcus and streptococcus and is used to identify [blank_start]hemolytic[blank_end] [blank_start]exotoxins[blank_end]
- Aenriched
- Bdifferential
- C5%
- Dfastidious
- Ehemolytic
- Fexotoxins
12Alpha hemolysis = [blank_start]partial[blank_end] lysis of red blood cells, results in a [blank_start]green[blank_end] halo around the colony (S. pneumoniae and S. viridians). Beta hemolysis = [blank_start]complete[blank_end] lysis of red blood cells, results in [blank_start]clear[blank_end] halo around the colony (S. pyogens and S. alagactiae). Gamma hemolysis = [blank_start]no[blank_end] lysis of red blood cells, results in [blank_start]no[blank_end] halo around the colony (E. faecalis and Strep bovis)
- Apartial
- Bgreen
- Ccomplete
- Dclear
- Eno
- Fno
13[blank_start]Chocolate[blank_end] agar is hemolyzed sheep blood and is used for growing [blank_start]fastidious[blank_end] organisms from the family [blank_start]Neisseria[blank_end] and Haemophilus
- AChocolate
- Bfastidious
- CNeisseria
14Mannitol salt agar is [blank_start]selective[blank_end] because it prevents all bacteria except [blank_start]staphylococcus[blank_end] from growing and [blank_start]differential[blank_end] because mannitol is fermented by S. aureus and growth with no mannitol fermentation is S. epidermis
- Aselective
- Bstaphylococcus
- Cdifferential
15CHROMagar is used for [blank_start]urine[blank_end] culture. It is [blank_start]differential[blank_end] and has enzyme [blank_start]substrates[blank_end], if a bacteria has the enzymes to utilize certain substrates a specific color develops. On a colony streak plate you can enumerate bacteria which is [blank_start]quantitative[blank_end] data and identify an isolate which is [blank_start]qualitative[blank_end] data
- Aurine
- Bdifferential
- Csubstrates
- Dquantitative
- Equalitative
16The enterotube II uses [blank_start]multi[blank_end] [blank_start]test[blank_end] [blank_start]media[blank_end] to identify gram [blank_start]negative[blank_end] [blank_start]rods[blank_end] in the family Enterobacteriaceae. The first step is a [blank_start]gram[blank_end] [blank_start]stain[blank_end] to isolate [blank_start]rods[blank_end] and the next is [blank_start]oxidase[blank_end] [blank_start]test[blank_end] to isolate [blank_start]oxidase[blank_end] [blank_start]negative[blank_end] organisms. Each compartment has [blank_start]differential[blank_end] media and it performs 15 biochemical tests.
- Amulti
- Btest
- Cmedia
- Dnegative
- Erods
- Frods
- Ggram
- Hstain
- Ioxidase
- Jtest
- Koxidase
- Lnegative
- Mdifferential
Information
- Description
- Quiz on Specialized media, created by Meagan Mueller on 12/12/2016.
- Author
- Meagan Mueller
- Language
- English
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