Flussdiagramm
DISAGGREGATION OF EXPLANT
Inhalt des Flussdiagramms
DISAGGREGATION OF EXPLANT
MECHANICAL DISAGGREGATION
ENZYMATIC DISAGGREGATION
EDTA TREATMENT
PRIMARY EXPLANT TECHNIQUES
DISSECTION
FINE CHOPPING
WASH BY SETTLING
TRANSFER TO BSS
REMOVE BSS
ADD THIN FILM OF MEDIUM
INCUBATE FOR 2-3 WEEKS
EXPLANT OUT GROWTH
TISSUE SAMPLE
CELL+DEBRIS
MORE CELL + DERBIS
VIABLE CELL+DERBIS
DILUTE THE CELLS
INCUBATE
PRIMARY ADHERENT CELLS CULTURE
SUB CULTURE
CELL LINE
CHOPPED
WASHED
COLD TRYPSINIZATION
WARM TRYPSINIZATION
TISSUE IN VIALS
TISSUE PIECES+100ml TRYPSIN
PIECES+TRYPSIN
PLACE ON ICE FOR 1.6 hr
DISAGGREGATE CELLS +TISSUE RESIDUES
DISPERSED CELLS IN MEDIUM
INCUBATE 35.6 DEG C
20-30 MIN
INCUBATE 35.6 DEG C
48-72 HR
DISPERSED CELLS
PRIMARY CELL CULTURE
COLLAGENASE
CENTRIFUGE
RESUSPEND
WASH
PRIMARY CELL CULTURE
STEPS INVOLVED IN CELL CULTURE
Informationen
- Beschreibung
- Flussdiagramm am DISAGGREGATION OF EXPLANT, erstellt von Keerthana Muruganandham am 26/12/2017.
- Erstellt von
- Keerthana Muruganandham
- Aufrufe
- 267
- Bewertung