Experimental Methods: Bio110-2016

Experimental Methods: Bio110-2016

1. Nucleotides

   • Do I want to know where they are made?
     • Yes
       • Pulse experiment... I know what radioactive precursor to use, right?
         • Autoradiography
           • Do I want to visualize the cell?
             • Microscopy... I know which kind I should be using, right? And how to prepare my cells or tissues?
               • Light or Flourescent Microscopy
               • Electron Microscopy
           • Do I want to differentiate between locations in the cell
             • Cell fractionization... I know which cell components separate at different speeds, right?
     • No, I want to know where they're going?
       • Pulse + Chase experiment
     • I just want to label them!

2. Proteins

   • Am I interested in a specific protein?
     • No, I'm interested in them all
     • Yes!
       • Well then I need an Antibody... I know how to make one, right?
         • Do I want to visualize where the protein localizes in the cell?
           • Fluorescent labeled antibody?
           • Gold labeled antibody?
         • Do I want to test which tissues, or individuals, or samples contain my protein?
           • Studying Tissues: Harvest Sample
           • Studying cell components: fractionization
             • Purify proteins. Use SDS
               • Gel Electrophoresis... before I visualize my protein of interest, I have to size separate all of them
                 • Western Blot
           • Studying individuals: sample individuals

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