Exam 2

Question 1

Air flow in a microbiology laboratory should be:

Accepted Answer

from lower to higher risk areas

Answer

minimized as much as possible

Answer

optimized to prevent the settling of dangerous aerosols

Answer

well-filtered by recirculating it through numerous filters before recycling

Question 2

Procedures such as grinding, mincing, vortexing, and preparing smears:

Accepted Answer

create aerosol droplets and should be performed only in an approved biosafety cabinet.

Answer

are hazardous and should be performed while wearing gloves and gown protection.

Answer

should be avoided with specimens known to contain infectious agents

Answer

produce a spill hazard and should be performed over an absorptive mat.

Question 3

Class I biological safety cabinets:

Accepted Answer

protect the environment from contamination by biological agents by filtering the air as it is exhausted from the cabinet.

Answer

sterilize the air as it passes over the material in the cabinet

Answer

are completely enclosed and have gloves that enable the worker to manipulate the material in the cabinet.

Answer

are often called laminar flow hoods.

Question 4

Optimal protection of specimen, personnel, and environment is accomplished with which class of biological safety cabinet?

Accepted Answer

Class IV

Answer

Class I

Answer

Class II

Answer

Class III

Question 5

The method of choice for sterilizing items such as antibiotic solutions, chemicals, radioisotopes, and vaccines that are heat-sensitive is:

Accepted Answer

filtration

Answer

dry, low temperature heat.

Answer

moist, low temperature heat.

Answer

chemical

Question 6

To be sterilized in an autoclave, infectious medical waste should be:

Accepted Answer

processed for 30 to 60 minutes at 132C and 15 psi pressure

Answer

packed tightly to avoid dangerous pressure pockets.

Answer

processed for 15 to 20 minutes at 121 C and 30 pounds per square inch (psi) pressure.

Answer

processed for at least 1 hour at 121 C and 15 psi pressure.

Question 7

The autoclave sterilizes material by:

Accepted Answer

applying moist heat at high temperatures and increased pressure.

Answer

applying dry heat at high temperatures and pressure.

Answer

infusing gaseous chemicals that kill organisms.

Answer

cleaving protein bonds found in most biological agent cell walls.

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