Flowchart
Develop of B cells
Flowchart content
Development of B cell
B cells are responsibles of the generation of antibodies
The development of them from an stem cell could be summarized throug 6 steps
Generation of B cell
Negative selection:
Positive selection
Searching for infection
Finding and Attack infection
kill self reactive Bcells
B cells compete for folicles in secondary lymphoid tissues
High endothelial venules (HEV)
lymph recirculation patroling
Repertoire assembly
Generation of diverse clonaly expressed B cells in the bone marrow
early pro B cells:DJ both chromosome rearragement
late pro B cells:V DJ both chromosome
Stem cell
heavy chains rearragemnt
Light chain rearrangement
Large pre B cell :mu heavy chain (inmunoglobulin) is being made
Small pre B cell
Immature B cell: IgM KAPPA/MU on surface
apoptosis
arragement ?
no
yes
Kappa arranged in 1 and 2 chromosomes ?
lambda arranged in 1 and 2 chromosomes?
yes
No
No
No
apoptosis
apoptosis
Negative selection
Symbology
Bone marrow
Periferial
self reactive?
No
Goes to blood
expresses IgD and IgM
Immature B cell: IgM LAMBDA/MU on surface
yes
keep in the bone marrow
receptor editing
continue modifying llight chains
self reactive?
No
Yes
Yes
Only CD10 PROTEIN
CD10, CD34,CD19,CD127 PROTEINS
VLA-4, VCAM-1 mantain attached the b cell to bone
IL 7 PROMOTE TO MATURATE
SEEMS LIKE B-CELL RECEPTOR (LAMDA 5 VPREB) SUBSTITUTES OF LIGHT CHAIN
1 check point
2 check point
enters to peripherial circulation
immature B cells must pass through a primary follicle i to become mature B cell
anergic
Possitive selection
Attract them by chemokines CCL21 and CCL19
Mature B cells expresses IgM AND IgD inmunoglobulins
Are segregated via lymphatic system
searching for infection
apoptosis
Finding and attack infection
Antigen activated by B lymphoblast
Alternative splicing
Somatic hypermutation
IgG switching
Antibody secretes plasma cells
IgG and IgM fight agaisnts the infectoion
After all
Memory cells
Author: Manuel Enrique Alvarez Del Rio
References: Parham, P. (2021). The Immune System: Fifth International Student Edition with Registration Card. WW Norton & Company.