Flashcards
slide prep - from biopsy to processing
Cards in this deck (9)
- 11. Biopsy types - the removal of tissue for histologyCore e.g. muscle
amputation e.g. leg
incision e.g. skin
open e.g. lung
pipelle e.g. endometrium - 22. Fixation - aimsAims to preserve tissue in as close to lifelike state as possible
- 3Fixation-jobs- block enzyme act by chemicals & heat
Chemicals easier
- allows further processing of samples
- stabilises tissue by arresting degenration - 4Fixative types - cross-linking (additive) - precipitation (non-)- formaldehyde (4% formaldehyde: 10% formalin)
- alcohols - 5formaldehyde pros cons- slow, reversible, cheap, restored colour & good for dissection
- Toxic, for CHCOOH in air, polymerised paraformaldehyde - 6factors affecting FixativesTemperature
Specimen
Volume
Concentration
Duration - 73. data entry- Must include correct data and filled up fully.
- full Name, D.O.B, NHS no. - 84. Tissue selection and description – Grossing/Cut up- cut up or trim tissue sample and store in cassette with bar code
- 95. tissue processing- microtomy and impregnate tissue in wax.
3 steps: dehydration, clearing & impregnation
automated
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- BIOL 282 - Cellular pathology Flashcards on slide prep - from biopsy to processing, created by Jumael Zafar on 12/11/2019.
- Created by
- Jumael Zafar
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