Flashcards
DG - lecture 5
Cards in this deck (35)
- 1desmosomesbind cell to cell jxns through cadherins (never thru intermediate filaments)
- 2epidermolysis bullosa results because ofa mutation in integrin - a6b4 connects epicells to the dermis. there's a mutation in the alpha 6 unit, resulting in blistering and erosions of the skin.
- 3what is the lamina densait's part of the basement membrane, right on top of the dermis
- 44 kinds of cell adhesion moleculesintegrin (hetero), selectin (hetero), IG superfam (homo), cadherins (homo)
- 54 properties affecting cell-cell adhesion strength1. binding affinity 2. spatial distribution 3. activity state of adhesion molecules 4. external forces surrounding the cells (ie edema prevents cells from getting close enough t/g for proteins to see e/o and bind)
- 6lateral vs intercellular bindinglateral is on the same cell, occurs before intercellular (b/w cells)
- 7what do anchoring jxns domechanically attach cells to neighbouring cells or the ECM
- 8what do occluding jxns doprevent small molecule passage
- 9what do communicating jxns dothe mediate the passage of ions/solutes or electrical signals from one cell to its partner
- 10cell-matrix exs of anchoring jxnshemidesmosomes (intermed filaments to ECM thru integrin)
focal adhesions (actin to ECM thru integrin) - 11cell-cell exs of anchoring jxnsadherens junctions (actin to actin b/w cells) ie made of E cadherin
desmosomes (intermediate filaments to intermediate filaments) ie made of desmoglein, desmocollin - 12where are occluding jxns aka tight jxns most commonly found?polarized epithelia ie small intestine, kidney tubules, BBB
- 13how do you tell if something is part of the occludin jxn complex?they have "dins" in suffix part of their name: claudins, occludins etc.
- 14what is paracellular transport?transport between cells, not through them
- 15claudins and occludinspart of the occludin junction complex so they're called "dins"
- 16claudinsproteins that allow or don't allow small molecules to get through
- 17claudin in proximal tubule tight junctionclaudin 2 in proximal, permits passage of K+ and H2O
- 18claudin in ascending limb of tight junctionclaudin 16 , permits passage of Mg2+
- 19claudin in distal tubeclaudin 8 prevents passage
- 20claudin in collecting tuctclaudin 4 prevents passage
- 213 kinds of communicating junctions1. channels that mediate passage of ions/solutes or electrical signals from one cell to its partner
2. gap jxns
3. connexins - 22size cap of communicating junctions1000 Daltons
- 23gap junctions are made up ofconnexins which make up connexons
- 24connexona hemichannel, 2 make up a gap junction
- 25structure between two cardiomyocytesintercalated disc, which is made of a series of junctions. desmosomes and adherens are the strong jxns that allow gap jxns to come t/g. no strong jxns = no gap jxns = no communication = arrhythmia
- 26arrythmogenic right ventricular cardiomypopathy (ARVC)happens when there's a mutation in the proteins that make up the strong jxn: desmoglein, plakoglobin, plakophilin, desmoplakin, desmocollin
- 27production of melaninmade by melanocytes which protect basal cells (replenish epithelium). melanocytes are triggered to release melanin when hit by UV light. the keratinocytes take up the melanin and put up a parasol of melanin on top of its nuclei
- 28where in the epidermis are tight junctions found most frequentlythe upper layers, stratum corneum and stratum granulosum
- 29what jxn is used to attach the basement membrane to the dermislaminin and anchoring fibril attached to integrin based adhesion
- 30what jxns are used to attach cells together in the epitheliumtight and actin assoc adherens
- 31how many subunits in a connexon6
- 32process of setting up 3d cultures of skin1. collect normal tissues and isolate the primary epithelial cells
2. single step multiplex gene transduction 4. transfer cells onto basement membrane w living stroma (ECM + fibroblasts) to establish 3d tissue - 33keratin and vollagen VII in 3d cultureskeratin = protein found in epidermis
collagen VII = protein found in basement membrane (like collagen 4) - 34movement of keratin in neoplastic tissuestarts to go into the dermis, past col 7
- 35—
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- midterm Cell Phys 3140 Flashcards on DG - lecture 5 , created by Mary Nguyen on 04/10/2015.
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