Flashcards
Eukaryotic Cell Junctions
Cards in this deck (14)
- 1How do we examine cell-cell junctions?Light and electron microscopy
Particularly conventional TEM and freeze fracture - 2Why use freeze fracture?Produces a gold replica rather than a section
- 3What is the conventional TEM procedure?Put cells into a block of resin
Slice the block of resin - 4What is the freeze fracture procedure?Freeze sample and hit at a specific orientation
Spray with gold - 5What are Tight junctions and where are they found?Found in internal epithelia
Create a complete cellular layer with well controlled passage of substances into and out of the cell to prevent contamination of unwanted substances - 6What enforces tight junctions?Cross links between adjacent cells
- 7What are the functions of tight junctions?1. Prevent leakage between cells
2. Create different cell surface domains - 8What are Anchoring Junctions?Junctions that provide the cell with mechanical strength and links the cytoskeleton between adjacent cells and to the ECM.
- 9What are the basic structural components of anchoring proteins?Cytoskeletal filament, attachment protein and a transmembrane linker protein
- 10What is a Desmosome?A type of anchoring junction where two adjacent cells are attached, formed from protein plaques (desmoplakin) in the cell membranes linked by intermediate filaments.
- 11What is Cadherin?An adhesion protein that binds cells to each other in the formation of adherens junctions
- 12What are Adherens junctions?A cell junction whose cytoplasmic face is linked to the actin cytoskeleton by actin filaments.
- 13What are Gap Junctions?A specialised intercellular connection that directly connect the cytoplasm of two cells, which allows various molecules, ions and electrical impulses to directly pass through a regulated gate between cells
- 14What are Connexons?An assembly of six proteins called connexins that form the hexameric cylindrical pores for a gap junction between the cytoplasm of two adjacent cells. Allow passage of ions and small soluble moelcules to pass through gap junctions.
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- University The Dynamic Cell Flashcards on Eukaryotic Cell Junctions, created by Olivia Hearn on 03/05/2019.
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- Olivia Hearn
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