Flashcards
H+E histology practical
Cards in this deck (11)
- 1Tissue stained with haematoxylin and eosin shows ... stained what colour?cytoplasm... pink
- 2issue stained with haematoxylin and eosin shows the... stained what colour?nuclei... Dark purple
- 3What is regressive staining?A type of staining in which tissues are overstained and excess dye then removed selectively until the desired intensity is obtained.
- 4What is progressive staining?progressive staining stains to desired intensity without initially overstaining
- 5What is eosin?-Eosin is an acidic dye
-negatively charged
-it stains basic structures red or pink. - 6What is Haematoxylin?-a basic dye
-positively charged
-it stains acidic structures a purplish blue. - 7Haematoxylin is not strictly a basic dye, but it is used with a 'mordant' that makes this stain act as a basic dye. What is the mordant?aluminium salts
- 8How does the mordant dye the nuclei?Binds to the tissue
- 9What is histoclear?Histoclear is used to clear tissues,it also removes paraffin wax so that they can be rehydrated and stained.
- 10What is the H&E method used for?it provides a very detailed view of the tissue. It achieves this by clearly staining cell structures including the cytoplasm, nucleus, and organelles and extra-cellular components and can help with diagnosis of diseases.
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- Flashcards on H+E histology practical , created by Katie-jae Dalton on 06/01/2020.
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- Katie-jae Dalton · Jan 2020
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- Jan 6, 2020
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