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Quantification of Protein: Lowry Method
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Quantification of Protein: Lowry Method
First Reaction
- Formation of Copper ion complex.
- Forms reduced Copper in alkaline
- Formation of Copper ion complex.
Second Reaction
- Reduction of Folin-Ciocalteu by tyrosine and tryptophan.
- Reduced reagent detectable in range 500 - 700 nm.
- Reduction of Folin-Ciocalteu by tyrosine and tryptophan.
Folin-Ciocalteu Reagent
- Makes assay x100 more sensitive.
- Sensitivity used: 0.01 - 1.0 mg/mL.
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- Chemical Biology and Enzyme (Chemical Biology) Mind Map on Quantification of Protein: Lowry Method, created by Clevanisty Clandisity on 05/12/2019.
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- Clevanisty Clandisity
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