Jm's project

Jm's project

1. Rep.MECH

   • Microarray
     • Rep. specific candidates
       • iPSCR
       • PDR
       • intermediate
         • LCE3 family
     • GO analysis
       • GSEA
       • Panther pie chart
   • VDR ligands
     • qRT-PCR confirm
       • Rpl7, Rsp14
     • Over during Rep. (2'MEF)
       • AP staining
       • ICC
       • qRT-PCR
   • 2'MEF prep.
     • Puro. selection
     • Stock freezing
     • Rep. Test

2. Rep2hiDP-AV

   • hSTEMCCA-AV
     • Amplification (293T)
       • Ultra centrifuge
     • Working test (CRL2097)
       • Rep. efficiency
         • ICC
       • Protein synthesis
         • Western Blot
   • hOK/SM-AV

3. ICTR-rep.

   • Reference searching

4. iPSeval

   • EB diff.
     • HSP0076 characterization
   • Immunogenicity
     • Monkey Fib culture
     • Monkey iPSC generation
       • Sendai virus
       • Episomal vector