89748
Description
Mind Map by hannah.lindridge, updated more than 1 year ago
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Created by hannah.lindridge
about 11 years ago
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Investigating
organelles
- Cell
fractionation
- Cold
- To reduce enzyme
activity which might
break down organelles
- To reduce enzyme
activity which might
break down organelles
- Isotonic
- Prevent
bursting/shooting by
osmostic loss/gain
- Prevent
bursting/shooting by
osmostic loss/gain
- Buffered
- To maintain constand pH
- To maintain constand pH
- Cold
- Homogenation
- Cells broken up by
homogeniser (blender)
- Resultant fluid (homogenate)
filtered to remove complete
cells and large debris
- Resultant fluid (homogenate)
filtered to remove complete
cells and large debris
- Cells broken up by
homogeniser (blender)
- Ultracentrifugation
- Tubes of homogenate
spun at high speed
- Tube of filtrate placed in
ultracentrifuge and spun at
low speed
- Heaviest organelle
forced to bottom
(sediment)
- Fluid at top (supernatant)
removed leaving only
sediment
- Supernatant transferred to
another tube and spun again
at higher speed
- Next heaviest organelles
forved to bottom- process
continued with increase in speed
each time
- Next heaviest organelles
forved to bottom- process
continued with increase in speed
each time
- Supernatant transferred to
another tube and spun again
at higher speed
- Fluid at top (supernatant)
removed leaving only
sediment
- Heaviest organelle
forced to bottom
(sediment)
- Tubes of homogenate
spun at high speed
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