Flashcards
Microbiology I
Cards in this deck (14)
- 1What did Louis Pasteur discover?Disproved spontaneous generation
Showed heat could sterilise
Showed atmosphere is full of MO - 2What did Robert Koch prove?That MO caused disease, forming Koch's postulate
- 3How did he do this?4 postulates
1. Suspect pathogen must be present in all cases of disease, absent in healthy
2. Suspect pathogen must be grow in pure growth culture
3. Cells from pure culture of suspect causes disease in healthy animals.
4. Suspect must be reisolated and look similar to OG sample. - 4What is a slope?Set agar in a tube, used for pure growth of a MO
- 5When do you use no agar tests?For liquid, used for blood tests
- 6What is the problem with Brightfield microscopy?Kills the MO
- 7How can this be avoided?Using Phase contrast and Darkfield microscopy which keeps sample alive
- 8What is DIC microscopy?Use of polarised light to make cellular structures appear more 3D.
- 9What is Atomic Force microscopy?Measured force between probe and atoms of cell's surface
Used to look at pili - 10What is Confocal Scanning Laser microscopy? (CSL)Use laser with a fluorescent microscope
Cells usually stained with fluorescent dyes - 11What is Electron microscopy?Electrons used instead of visible light
Whole system operates in a vacuum - 12What is Transmission Electron microscopy?TEM is high magnitude and high resolution
Requires a thin slice of specimen as electrons can't penetrate tissue - 13What is scanning electron microscopy?Specimen coated in a heavy metal i.e. Gold and visualised by electron scatter
- 14What types of MO exist? (7)Basteria
Archaea (2 phylum)
Protozoa
Algae
Prions
Viruses
Fungi
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- User has deleted their subject information Flashcards on Microbiology I, created by Deleted user on 21/01/2018.
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- Deleted user · Jan 2018
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- Jan 21, 2018
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- Created by J yadonknow · Jan 21, 2018
- Copied by J yadonknow · Jan 21, 2018
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