Flashcards

Cell Signalling II

Deleted user User has deleted their subject information English 18 cards

Cards in this deck (18)

12' Messengers + signal transductioncAMP
PKA
cGMP
PKG
2Ligand-induced production of cAMPProduced from ATP by effector enzyme adenylyl cyclase
3Adenylyl Cyclase2 transmem domains joined by a cat IC domain
ATP degraded to 5'MP by a cAMP phosphodiesterase
4cAMP mediatorPKA
5Guanylyl Cyclase functionConverts GTP into diffusable cGMP
62 formsMembrane bound - + by external L
Soluble cytoplasmic form* -
+ by intrinsic mol. e.g. NO
7How is cGMP degradedDegraded by cGMP phosphodioesterase
8PIP2 +PiPIP2 -> PIP3 via PL3K
PIP3*->PDK1->PKB-> Cell proliferation
9PIP3*->PIP2Via PTEN - a tumour surpressor
10Breakdown of PIP2 in LBLPLC splits PIP2
DAG-> PKC
InsP3-> Ca2+ release
11How does Ca2+ act as a 2' messenger?Δ in [Ca2+] in cytosol constitutes the signal
12^ [Ca2+]?Influx of EC Ca2+
Release from IC stores e.g. ER/SR/Mito
13Decrease [Ca2+]?Ca2+ pumps sequester it back into organelle (remember the conc. in cytosol constitutes the signal).
14Structure and function of Calmodulin1. 4 Ca2+ binding sites
2. + When [Ca2+]>500nM
3. Ca-Calmodulin can bind + modify activity of target e.g. Calmodulin-dependent protein kinases (CAM Kinases)
15Roles of CAM Kinases (4)Regulates fuel metabolism
Muscle contraction
Cell division
Inflammatory response
16Kd[L] where 1/2 R is bound
Measure of binding affinity
17Kd formula[R][L]/[RL]
18Deriving Kd from binding assayTotal Binding (A) - Nonspecific Binding (B)
=
Specific Binding (C)
1/2 [R] bound = Kd

Description: User has deleted their subject information Flashcards on Cell Signalling II, created by Deleted user on 31/03/2018.
Created by: Deleted user · Mar 2018
Updated: Mar 31, 2018
Views: 14
History: Created by J yadonknow · Mar 31, 2018

Copied by J yadonknow · Mar 31, 2018
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