Flashcards
Extra-Chromosomal Genetics II
Cards in this deck (18)
- 1How do chromosomal genes end up in plasmids?Reciprocal strand exchange at sites of homology between insertion sequences, homo reco.
- 2What do transposase enzymes doMove the gene as a TRANSPOSON
- 3How do transposons encourage genetic diversity?Allow homologous recombination
- 4How is this process used during DNA replication repair?Repairs stalled replication forks by resolving Holliday junctions when they arise
- 5What is the name of a plasmid that has been inserted into a genome?integrated plasmid
- 6What is an episomal plasmid?A plasmid capable of reversible integration into a chromosome
- 7How can a prokaryotic plasmid and chromsome exhibit diploid characteristics? (4)1. Plasmid picks up Gene1 from chromosome via excision.
2. Conjugation with another cell occurs.
3. Homology with chromosomal allele of Gene1 in recipient (little X).
4. HR can occur, recipient cell essentially becomes diploid for the region of chromosome containing Gene1. - 8How do conjugative plasmids unravel chromosomes? (2)An integrated OriT will mobilise both the plasmid AND the chromosome
It still has tra genes and will encode a pili and try to join to F- cells - 9How does this increase the amount of G. variation?As the chromosome now becomes fully available for HR.
- 10Why does the transfer of whole chromosomes rarely occur?As the conjugation pili is prone to breakage.
- 11What name is given to cells that have integrated conjugative plasmids?Hfr strains
- 12What is Transduction?Chromosomal DNA movement between cells mediated by a prokaryotic virus.
- 13What is general transduction?Viral mediated GI transfer
- 14Outline the steps of a lytic lifecycle. (6)1. Attachment
2. Injection of viral nuc. acid.
3. N. acid rep.
4. Viral capsid synth.
5. Viral assembly.
6. Cell lysis + virion release - 15MOA of transduction (3) Eat Pack Sex1. Digestion
Host Chr DNA with nuclease.
2. Encapsulation
Host Chr DNA into capsid and incorporation into viral genome.
3. Undergo HR
w/ new host cell at sites of homology, as opposed to lytic infection. - 16What is special transduction?If host cell is exposed to cell-stress, then Lambda virus will leave, accidentally pack host Chr DNA with it.
- 17What is transformation?Deliberate uptake of external DNA
- 18How can you force competence in E. Coli?Treat w/ 100mM CaCl2 and 15s heatshock.
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- User has deleted their subject information Flashcards on Extra-Chromosomal Genetics II, created by Deleted user on 21/04/2018.
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- Deleted user · Apr 2018
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- Apr 21, 2018
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- Created by J yadonknow · Mar 8, 2018
- Copied by J yadonknow · Apr 21, 2018
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