Flashcards
122 - Stains & Properties of Growth Media
Cards in this deck (33)
- 1What is the mnemonic device/sentence to remember the bugs that do not Gram stain well?These Little Microbes May Unfortunately Lack Real Color But Are Everywhere
- 2Which bugs do not Gram stain well because they are too thin to be visualized?Treponema; Leptospira
Mnemonic device: These Little Microbes May Unfortunately Lack Real Color But Are Everywhere - Treponema (These); Leptospira (Little) - 3Why do the following not Gram stain well? Treponema; LeptospiraThey are too thin to be visualized
- 4Which bugs do not Gram stain well because the cell wall has high lipid content?Mycobacteria
Mnemonic device: These Little Microbes May Unfortunately Lack Real Color But Are Everywhere - Mycobacteria (Microbes) - 5Why does the following not Gram stain well? MycobacteriaThe cell wall has high lipid content
- 6Which bugs do not Gram stain well because there is no cell wall?Mycoplasma, Ureaplasma
Mnemonic device: These Little Microbes May Unfortunately Lack Real Color But Are Everywhere - Mycoplasma (May), Ureaplasma (Unfortunately) - 7Why do the following not Gram stain well? Mycoplasma, UreaplasmaNo cell wall
- 8Which bugs do not Gram stain well because they are primarily intracellular?Legionella; Rickettsia, Chlamydia, Bartonella, Anaplasma, Ehrlichia
- 9Why do the following not Gram stain well? Legionella; Rickettsia, Chlamydia, Bartonella, Anaplasma, EhrlichiaPrimarily intracellular
Also: Chlamydia lack classic peptidoglycan because of decreased muramic acid. - 10What is the mnemonic device / phrase for remembering Giemsa Stain?Certain Bugs Really Try my Patience
- 11What are Giemsa stains used for?Chlamydia, Borrelia, Rickettsia, Trypanosomes, Plasmodium
- 12What are Periodic acid-Schiff stains used for?Stains glycogen, mucopolysaccharides; used to diagnose Whipple disease (Tropheryma whipplei)
- 13Which stain is used for the following? Stains glycogen, mucopolysaccharides; used to diagnose Whipple disease (Tropheryma whipplei)Periodic acid-Schiff stain
- 14Which stain is used for the following? Chlamydia, Borrelia, Rickettsia, Trypanosomes, PlasmodiumGiemsa stain
- 15What is the mnemonic phrase used to remember Periodic acid-Schiff stain?PaSs the sugar
- 16What are Ziehl-Neelsen stains (carbol fuchsin) used for?Acid-fast bacteria (eg Mycobacteria, Nocardia; stains mycolic acid in cell wall); protozoa (eg Cryptoporidium oocysts)
- 17Which stain is used for the following? Acid-fast bacteria (eg Mycobacteria, Nocardia; stains mycolic acid in cell wall); protozoa (eg Cryptoporidium oocysts)Ziehl-Neelsen stains (carbol fuchsin)
Current standard of care is auramine-rhodamine stain for screening (inexpensive, more sensitive but less specific) - 18What are India ink stains used for?Cryptococcus neoformans; mucicarmine can also be used to stain thick polysaccharide capsule red
- 19Which stain is used for the following? Cryptococcus neoformans; mucicarmine can also be used to stain thick polysaccharide capsule redIndia ink stains
- 20What are Silver stains used for?Fungi (eg Coccidioides, Pneumocystis jirovecii, Legionella, Helicobacter pylori
- 21Which stain is used for the following? Fungi (eg Coccidioides, Pneumocystis jirovecii, Legionella, Helicobacter pyloriSilver Stains
- 22What are Fluorescent stains used for?Used to identify many bacteria and viruses
Example is FTA-ABS for syphilis - 23Which stain is used for the following? Used to identify many bacteria and viruses Example is FTA-ABS for syphilisFluorescent antibody stain
- 24What is the first-line lab test in bacterial identification?Gram Stain
- 25With a gram stain, bacteria with thick peptidoglycan layer retain what dye?Crystal Violet Dye (Gram+)
- 26With a gram stain, bacteria with thin peptidoglycan layer turn what color?Red or Pink (Gram-) with counterstain
- 27What is Selective Media?Favors the growth of particular organism while preventing growth of other organism.
For example: Thayer-Martin agar contains antibiotics that allow the selective growth of Neisseria by inhibiting the growth of other sensitive organisms. - 28The following is an example of what? Thayer-Martin agar contains antibiotics that allow the selective growth of Neisseria by inhibiting the growth of other sensitive organisms.Selective Media
- 29What is Indicator (differential) media?Yields a color change in response to the metabolism of certain organisms.
Eg: MacConkey agar contains a pH indicator; a lactose fermenter like E coli will convert to acidic metabolites leading to color change - 30The following is an example of what? MacConkey agar contains a pH indicator; a lactose fermenter like E coli will convert to acidic metabolites leading to color changeIndicator (differential) media
- 31What gram stain is used for the following? ChlamydiaGiemsa stain
- 32What gram stain is used for the following? Whipple disease (Tropheryma whipplei)Periodic acid-Schiff stain
- 33—
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- User has deleted their subject information Flashcards on 122 - Stains & Properties of Growth Media, created by Deleted user on 15/05/2017.
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- Deleted user · May 2017
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- May 15, 2017
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- Created by Victoria Wright · May 10, 2017
- Copied by Victoria Wright · May 15, 2017
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