13592079
Descripción
Test por Jessica Bulley, actualizado hace más de 1 año
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Creado por Jessica Bulley
hace más de 8 años
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Pregunta 1
Pregunta
Define Histopathology
Respuesta
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the study of changes in tissues caused by disease.
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the study of changes in cells caused by disease.
Pregunta 2
Pregunta
Select Three hazards associated with working in a Histopathology laboratory.
Respuesta
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- Chemical: fixatives, stains, chemicals
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– Biological: cross infection
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– Physical: sharps, radiation
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- Safety: slips, trips, falls
Pregunta 3
Pregunta
Explain what a fixative does
Respuesta
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preservation of tissue specimen
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decontaminates all viruses associated with the tissue specimen
Pregunta 4
Pregunta
List Six features of an ideal fixative
Respuesta
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• Will not swell or shrink tissue
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• Is economical
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• Has convenient shelf life and storage
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• Will be safe for user and environment
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• Will preserve tissue in life like manner
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• Will not add artefact material to tissue
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• Will add artefact material to tissue
Pregunta 5
Pregunta
Select Three fixative/s that can be used for Cytology smears?
Respuesta
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Spray fixed with aerosol of propanol and acetone+ Poly ethylene glycol- a water soluble wax
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Methanol
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Ethanol
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Xylene
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Sodium dihydrogen phosphate monohydrate 4g
Pregunta 6
Pregunta
Select Three physical agents that can be used to assist in the fixation of tissues.
Respuesta
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Microwave
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Heat
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Ultrasound
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Freeze
Pregunta 7
Pregunta
True or false: autolysis represents the changes to tissue that occur before fixation due to bacterial contamination.
Respuesta
- True
- False
Pregunta 8
Pregunta
What type of fixative is Neutral Buffered Formalin classed as?
Respuesta
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Universal fixative
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Fixative used for specific purposes
Pregunta 9
Pregunta
Why is Formalin Buffered to a neutral pH?
Respuesta
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because the further a fixative is from the pH of body tissues the more distortion you can get in the tissue at the cellular level.
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because the further a fixative is from the pH of body tissues the less distortion you can get in the tissue at the cellular level.
Pregunta 10
Pregunta
What is the function of xylene in tissue processing?
Respuesta
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To cut sections, the tissue has to be embedded in paraffin wax, but wax is not soluble in water or alcohol. However, it is soluble in a paraffin solvent called 'xylene'.
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Cleaning up the lab station to prevent contamination of other specimens and users of the facilities
Pregunta 11
Pregunta
Why is the histological section cut to approximately 5µm thick?
Respuesta
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- Sample needs to be thin enough for light microscopy (about 1 cell layer thick) - makes it easier to evaluate tissue and look at its morphology
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- Provides enough thickness to account for cell to cell ratio
Pregunta 12
Pregunta
List method/s used for the collection of Cytology samples.
Respuesta
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- Pap Smears, Endoscopy samples, some FNA (fine needle aspiration) specimens
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- Blood test, saliva test, sputum collection
Pregunta 13
Pregunta
Give an example of a Chemical Fixative that belongs to Aldehydes
Respuesta
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- Formaldehyde, Glutaraldehyde
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- Ethanol, Methanol
Pregunta 14
Pregunta
Give an example of a Chemical Fixative that belongs to Oxidising agents
Respuesta
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- Osmium tetroxide, Potassium Dichromate
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- Ethanol, Methanol
Pregunta 15
Pregunta
Give an example of a Chemical Fixative that belongs to Protein coagulants
Respuesta
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- Ethanol, Methanol
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- Mercuric Chloride, Picric acid
Pregunta 16
Pregunta
Give an example of a Chemical Fixative that belongs to Uncertain mechanism
Respuesta
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- Mercuric Chloride, Picric acid
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- Ethanol, Methanol
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