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IDI
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- 1What are Falkow's PostulatesThe potential virulence factor should be found in all pathogenic strains but be absent from non-pathogenic relatives.
Specific inactivation of the gene(s) should attenuate virulence in an appropriate animal model
reintroduction of gene restores virulence - 2Name 3 methods of horizontal gene transferTransformation - cells pick up naked DNA from the environment
Transduction - viruses move DNA between cells
Transconjugation - cells donate DNA to recipient cells via pili - 3What two states does a plague exist in nature?Enzootic - Stable infection cycle in a relatively resistant host population without excessive host mortality
Epizootic - plague bacilli have been introduced to moderately or highly susceptible mammals. Massive die-offs occur, causing parasite to seek other hosts - 4Name the method used to study the virulence genes involved in the evasion strategyGentamicin Protection Assay
- 5Name the 3 steps of the Gentamicin Protection Assay
- 6What is the importance of Complementation?Complementation allows researchers to determine that the
gene knockout is responsible for the observed attenuation of
the isogenic mutant and not due to a polarity effect - 7How does Complementation work?The removed gene is reintroduced to the isogenic mutant through the use of plasmid which contains the functional gene.
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- Bsc hons Biological Sciences Fichas sobre IDI, creado por Jake Costello el 22/02/2016.
- Creado por
- Jake Costello
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