Microscopy

Microscopy

1. Light microscopy

   • up to x1500
     • 200nm
       • visualize cells+major intracellular organelles
         • 2D but sometimes can be 3D
           • live specimen, cell movement+motility
             • Low

2. Transmission electron microscopy

   • up to x200 000
     • 0.2nm
       • details of intracellular organelles
         • 2D
           • electrons are transmitted through the speciman, so it has to be dead+fixed
             • high

3. Scanning electron microscopy

   • wide range (from x10 to x100 000)
     • 1nm
       • image of surfaces
         • 3D
           • electrons are reflected back from the surface of the speciman
             • high

4. Microscopys

   • Magnification
     • Resolution
       • what is it used for?
         • Dimensions
           • Speciman
             • Cost