Microscopy

elisemaybishop
Mind Map by elisemaybishop, updated more than 1 year ago
elisemaybishop
Created by elisemaybishop over 6 years ago
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A Levels Biology (Cells) Mind Map on Microscopy, created by elisemaybishop on 06/25/2014.

Resource summary

Microscopy
  1. Magnification & Resolution
    1. Magnification is how much bigger it appears under the microscope than it is in real life
      1. MAGNIFICATION = IMAGE SIZE + ACTUAL SIZE
      2. The term resolution refers to the ability to see two distinct points separately
      3. The light microscope
        1. Light passes from a bulb under the stage...
          1. ...through a condenser lens...
            1. ...and then through the specimen
              1. This beam of light is passed through an objective lens and then the eyepiece lens
          2. OVERALL MAGNIFICATION = OBJECTIVE LENS MAGNIFICATION x EYEPIECE LENS MAGNIFICATION
            1. Some specimens have to be prepared before being viewed under the telescope
              1. 1. STAINING: coloured stains are chemicals that bind to chemicals in the specimen that allow the specimen to be seen
                1. 2. SECTIONING: specimens are embedded in wax - thin sections are then cut out without distorting the specimen
            2. The Electron Microscope
              1. Higher resolution
                1. Generate a beam of electrons that can distinguish objects at 0.2 nm apart
                2. TRANSMISSION ELECTRON MICROSCOPE
                  1. Electron beams pass through a very thin prepared sample and electrons pass through denser parts less easily
                  2. SCANNING ELECTRON MICROSCOPE
                    1. The electron beam is directed onto a sample. The electrons do not pass through the specimen, they bounce off
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