Flashcards
Urinaylsis
Cards in this deck (80)
- 1Four methods of Urine CollectionPatient Voiding
Manual Expression
Urinary catheterization
cystocentesis - 2Voided Collection (Hospitalized Animal)animal on raised grate
after voiding,collect from cage floor with syringe
Metabolism cage made with slanted floor to funnel voided urine - 3collection devices of void urinepaper cup,specimen cup, ladle etc.
- 4Voided Collectiona.k.a "free catch"
For routine UA
Bacteria,cells and debris (hair,skin & urethra)
initial portion > concentration of contaminants
use midstream catch if possible
cleanse vulva or prepuce before collection
use two containers (initial and midstream) - 5Voided Collection (Cats)Litter pan cover with plastic bag
if cat prefers to urinate in litter type material (NoSorb & Strips of wax paper)
Pads to capture urine, carpet, diapers, shredded newspaper, etc.(not ideal) - 6Manual Expressionusually done on cats
adequate for routine UA
may be difficult in males
may be difficult or easy w/ neurological impairment
never attempt w/ suspected obstruction - 7Catheterizationsuitable for culture
relieves urethral obstruction
empty bladder
aseptically and atraumatically as possible
easy aspirate into syringe attached to exposed end of catheter
pass rubber ,plastic or metal tube through urethra into bladder
type and size depend on sex/species - 8Drawbacks of catheterizationurethral inflammation
introduce UTI
must be aseptically performed - 9Cystocentesispercutaneous aspiration of urine from bladder
used empty bladder if cannot catheterize
can use for bacterial culture and sensitivity
aseptic technique - 10Client-Collected Samplesusually unsatisfactory
extended periods of time between collection & testing
discourage pet from voiding for 2-3 hours before collection at clinic - 11Sample Perseverationshould be analyzed within 30 mins
refrigerate additional 6-12 hours
centrifuge immediately for cytological evaluation
can be preserved by adding formalin, toluene, or phenol(changes may occur to analyses) - 12Sample Preservation Degradation of urine components overtime (Increases)Ammonia
Bacteria
hemolyzed blood
pH (may decrease)
protein (may decrease) - 13Sample Preservation Degradation of urine components overtime (decreases)bilirubin
ketones
glucose
WBC
urobilinogen - 14Sample Preservation Degradation of urine components overtime (disappear )casts
- 15Sample Preservation Degradation of urine components overtime (darkens)color
- 16Sample Preservation Degradation of urine components overtime ( hemolyze)Erythrocytes
- 17Sample Preservation Degradation of urine components overtime (appear)Nitrites
- 18Sample Preservation Degradation of urine components overtime (develops)Turbidity
- 19Urinalysis (Indications )polydypsia
polyuria
dysuria
stranguria
pollakiuria
hematuria
periuria - 20physical properties of urinecolor
clarity
pH
volume
SG - 21chemical constituents of urineprotein
glucose - 22Components of UAphysical
chemical
sediment - 23UA (color) Yellow/amberdue to uroblilin
- 24UA (color) Affected byconcentration
food
dyes
blood - 25UA (color)usually correlates with SG
lighter urine = lower SG
Darker urine = higher SG - 26UA (color) yellow-brown to greenish foamcontains bile
- 27UA (color) red or reddish-brownhematuria
hemoglobinuria
some species normally have orange to reddish-brown urine (i.e. rabbits) - 28UA (clarity)normal clear/transparent
- 29UA (clarity) cloudycellular debris
epithelial cells
alkaline urine w/ crystal precipitation
UTI-bacteria ( often concurrent w/ alkalinity and WBCs)
casts,mucus,semen,lipids
on standing urine becomes more cloudy w/ increased bacteria & crystal formation - 30UA (clarity) Species with normally turbid urinehorse-calcium carbonate crystals & mucus
rabbit,hamster & guinea pigs-calcium salts
cattle urine will form calcium carbonate crystals on standing - 31UA (Odor)ammonia odor
fruity odor
foul odor - 32UA (odor) Ammoniaold specimen & bacterial proliferation
- 33UA (odor) Fruity odorketones
- 34UA (odor) Foul odorurinary tract infection
- 35UA (odor)strong odor typical of mice,tom cats, goats and pigs
- 36UA (specific gravity) Normal dogs1.025 (1.018-1.045)
- 37UA (specific gravity) Normal cats1.030 (1.020-1.040)
- 38UA (specific gravity) Normal equine1.035
- 39UA (specific gravity) Normal cattle & swine1.015
- 40UA (specific gravity) Normal sheep1.030
- 41UA (specific gravity) Fixed SGloss of both concentrating and diluting function
maintenance of constant osmolality or urine regardless changes in osmotic pressure of blood - 42UA (specific gravity) Isosthenuriafixed SG
- 43UA low SGdiabetes insipidus impaired ADH
loss concentrating ability (renal disease)
increased fluid intake - 44UA (specific gravity) hypostenurialow SG
- 45UA (specific gravity) hypersthenuriahigh SG
- 46UA (specific gravity) high specific gravityadrenal insufficiency (impaired aldosterone)
hepatic disease
congestive heart failure
dehydration
decreased fluid intake - 47Hypersthenuria (cats)SG >@1.035
- 48Hypersthenuria (dogs)SG >@1.030
- 49Hypersthenuria (large animals)SG>@1.025
- 50UA (normal volume)1-2 ml/kg/hour
- 51UA Polyuriaincreased daily urine output
increased fluid intake
diuretics
nervousness/anxiety - 52UA Oliguriareduced daily urine output
decreased fluid intake or excess fluid loss
increased salt ingestion
renal shutdown
obstruction of urine flow - 53UA Anuriano urine production
renal shutdown
usually associated with obstruction - 54UA (pH)used to assess acid base balance
pH expresses hydrogen ion concentration or acidity
reagent strips most commonly used
often affected by diet - 55UA pH reagent stripsmethyl red
bromthymol blue - 56UA (pH) herbivoresalkaline 7-8.5
- 57UA (pH carnivores)6-7
- 58UA (pH omnivores)have either alkaline or acidic
- 59UA (pH acidic urine)high protein diet
acidosis /uncontrolled diabetes mellitus - 60UA (pH alkaline urine)after meals -alkaline tide
high vegetable diets
UTI
renal tubular acidosis - 61Renal tubular acidosisaccumulation of acid in the body due to failure of kidneys to acidify urine
- 62two reasons why renal tubular acidosis occurstubules do not reabsorb enough bicarbonate from filtrate PCT
or
tubules may not secrete enough H+ ions into the filtrate in DCT - 63UA (pH) Higher readingsloss of CO2 samples w/ left open and standing at room temperature
standing urine containing urease-producing bacteria - 64UA (pH ) Lower readingshighly acidic urine a false reduction of pH may result b/c dripping from the protein reagent pad onto the pH reagent pad
- 65Glucose: Glucosuria or Glycosuriablood levels of glucose exceed renal threshold @ 200 mg/dL
- 66Glucose testenzymatic tests specific for glucose
- 67Glucose exceeding 200 mg/ dL indicatesrenal glucosuria
diabetes mellitus increased volume with high specific gravity as an attempt to rid blood of excess glucose - 68ketonuriainadequate carbohydrates in diet or defects in carbohydrate metabolism
as a result increased fat metabolism leads to formation of ketone bodies
caused by diabetes mellitus - 693 ketone bodiesacetones , acetoacetic acid & b-hydroxybutyric acid
all present in same relative concentration - 70other body conditions that cause formation ketonessevere anorexia & starvation
- 71ketogenesisoccurs in hepatocyte mitochondria
- 72Rothera's Testnitroprusside + acetone or acetoacetic
- 73two methods of detecting proteinuriacolorimetric = dipstick method
precipitation method = sulfosalicylic acid (SSA) - 74Detecting Proteinuria colorimetric method (dipstick)usually tetrabromophenol blue on colorimetric reagent strip
- 75Detecting proteinuria SSA lab test procedure1. centrifuge urine
2. add 2.5 ml of supernatant to 7.5 ml of 3% sulfosalicylic acid
3. degree of turbidity correlates to protein - 76Detecting proteinuria clear0mg/dL
- 77Detecting proteinuria opalescent1-10 mg/dL
- 78Detecting proteinuria can read print through tube5-30 mg/dL
- 79Detecting proteinuria degree of turbidity = protein can read only black ines40-100 mg/ dL
- 80Detecting proteinuria no visible black lines150-500 mg / dL
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- Flashcards on Urinaylsis, created by Pandalover123 on 24/07/2014.
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