Flashcards
Chem: Chromatography
Cards in this deck (14)
- 1POP- Persistent Organic Pollutants
- Toxic to living organisms
- Long time to break down
- Most non-polar
- Cause damage to nervous system - 2Chromatography- Separation of molecules in a mixture
- Separation based on differences in structure and composition - 3Mobile Phase- Moves molecules
- 4Stationary phase- Doesn't move
- 5Intermolecular forces- Adsorption (Stick on stationary)
- Desorption (Unstick into liquid) - 6Adsorption- Depends on polarity of stationary phase and substance
- Hydrophilic will stick to hydrophilic - 7TLC- Stationary: Thin layer of powder on glass
- Dissolve pure substance
- Dissolve sample
- Place spots from solution at bottom of thin-layer plate
- Place plate when dry in solution
- Solvent rise until top and remove
- Examine with UV - 8Rf- Same under identical conditions
Affected by:
- Temperature
- Polarity of stationary
- Water vapor
- Type of solvent - 9Column chromatography- Solid stationary phase packed into a glass column.
- The sample is applied to the top.
- The mobile phase is dripped slowly onto the column.
- The different components elute from the bottom. - 10HPLC Process- Like column
- Small particle size of stationary
- More adsorption/desorption
- Mobile applied under pressure
- Detector used at end - 11HPLC- Testing medicine
- Analysing products (food, insecticide)
- Polymer analysis
- Detecting drugs - 12Chromatogram- Horizontal axis is (Rt)
- Most strongly adsorbed = longest Rt
- Least strongly adsorbed = Short Rt
- Larger molecules adsorb more strongly and take longer to elute - 13Rt- Same if identical conditions
- Used to identify components associated with a peak
- Temp
- Polarity of stationary
- Pressure of mobile - 14Qualitative analysis- Area underneath a peak is directly proportional to concentration of an individual component.
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- Flashcards on Chem: Chromatography, created by Aidan Wong on 16/11/2017.
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- Aidan Wong
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