Flashcards
Histology
Cards in this deck (21)
- 1What is histology?The study of MICROSCOPIC anatomy
- 2What is a tissue?A collection of similar cells from the same origin that work together to carry out a specific function
- 3***** What are the 4 types of tissue?1. Epithelium
2. Connective Tissue
3. Muscle
4. Nervous - 4What are 5 things to consider when analyzing a histological section?1. Magnification (high or low)
2. Type of stain
3. Overall organization
4. 2D vs 3D (what plane the slice was taken)
5. Type of microscope - 5The only microscope that can show 3D pictures is an ______.Scanning electron microscope (SEM)
- 6Proteins/cytoplasm are _____ and therefore bind ______ dyesBASIC, ACIDIC
- 7The nucleus/nucleolus is _____ and therefore binds _____ dyesacidic, BASIC
- 8Hemotoxylin is a _____ stain and stains _______. Eosin is a _____ stain and stains ______ (colour).Basic, purple
acidic, red/pink - 9Glandular excretion is _____ and therefore stains ____ (colour) with an H&E Stain.BASIC, red
- 10Usually, with an H&E Stain, the cytoplasm should be _____ however, is protein synthesis is occuring it will be _____ because ______.red usually
purple --> b/c amino acids are being combined during protein synthesis - 11A Verhoff stains ____ fibres. H&E cannot stain these.ELASTIC
ie) vasculature - 12Alcian blue stains ______.MUCUS
- 13Osmium tetroxide stains ______ which is found in ______.MYLEIN; nervous tissue
- 14The brain of someone with Parkinson's will have _____ neurons in the substantia nigra of the midbrain. There will be ____ staining compared to a normal patient.fewer, less staining
- 15What is extraction?Occurs when certain parts of a tissue are removed from a sample due to processing
- 16What are two types of molecules that are typically extracted from samples?Lipids and Mucins
- 17_____ stain prevents the extraction of mucins unlike H&EAlcian Blue
- 18An example of a "natural" space is in a normal vs. _____ muscle the ______ muscle has INCREASED space between cellsATROPHIED
- 19_____ is common in nervous tissue samples.SHRINKING
- 20What happens (sometimes) to the axons of nervous tissue during fixation?They appear to have longitudinal waves (from shrinking) even though they should be straight.
- 21***In samples, the connective tissue layer and the epithelial layer should _____ be joined.ALWAYS
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- DMED 511 - Foundations (Full Deck - PreMidterm) Flashcards on Histology, created by Manisha BHARADIA on 22/09/2018.
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- Manisha BHARADIA
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- Created by Manisha BHARADIA · Sep 22, 2018
- Copied by Manisha BHARADIA · Sep 22, 2018
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