Flashcards
MIBO 3510L Final practical
Cards in this deck (46)
- 1Columbia CNA w/ 5% Sheep's BloodSelective FOR Gram positives (colistin and nalidixic {Antibiotics}
Differential for hemolysis (sheep's blood)
Often used to ID Staph-, Strep-, and Enterococci - 2Mannitol Salt AgarSelects FOR Staphylococci (NaCl)
Differential for mannitol fermentation (phenol red pH indicator)
Often used to ID Staph. aureus - 3Bile Esculin Agarselects FOR intestinal organisms (bile salts)
differential for esculinase enzyme (esculin + ferric citrate)
often used to ID Enterococci and Group D Streptococci - 4MacConkey Agarselects AGAINST Gram positives (crystal violet)
selects FOR intestinal organisms (bile salts)
differential for lactose fermentation (neutral red pH indicator)
often used to ID E. coli and fecal coliforms - 5Eosin Methylene Blueselects AGAINST Gram positives (methylene blue)
differential for lactose fermentation (methylene blue and Eosin Y pH indicator)
often used to ID E. coli and fecal coliforms - 6Xylose Lysine Desoxycholateselects FOR Gram negatives (sodium desoxycholate)
differential for sugar fermentation (high lactose, high sucrose, low xylose) (phenol red PH indicator)
differential for lysine decarboxylation
differential for H2S production
often used to ID Salmonella species - 7N Free Brothtests for ammonia
N fixation - 8Ammonium brothtests for ammonia, nitrate, and nitrite
nitrification - 9peptone watertests for ammonia
ammonification - 10Nitrate brothtests for nitrate and gas production
denitrification - 11IMViC testsindole (+/- tryptophanase activity that produces indole)
Methyl Red (mixed acid products from glucose fermentation)
Voges-Proskauer (2,3-butanediol product during glucose fermentation)
Citrate (can citrate be used as sole C source) - 12Lysine Iron AgarUsed to ID Gram-negative enteric pathogens
differential for glucose fermentation
differential for H2S production
differential for lysine decarboxylation
differential for lysine deamination - 13LIA results: purple slant/purple buttdeaminase negative
decarboxylase positive - 14LIA results: purple slant/yellow buttdeaminase negative
decarboxylase negative
glucose fermentation occurred - 15LIA results: red slant/yellow buttdeaminase positive
decarboxylase negative
glucose fermentation occurred - 16intracellular enzymescatalase
oxidase
urease - 17extracellular enzymesamylase
DNase
hemolysins
casease - 18Triple Sugar Irondifferential for fermentation (glucose [low amount], sucrose, and lactose)
differential for H2S production
differential for gas production - 19TSI results: yellow slant/yellow buttglucose + sucrose and/or lactose fermentation
- 20TSI results: red slant/yellow buttglucose fermentation, proteins catabolized aerobically in slant with alkaline products [reversion]
- 21TSI results: red slant/red buttno fermentation; proteins catabolized aerobically in slant and anaerobically in butt with alkaline products
- 22TSI results: red slant/no change in buttno fermentation; proteins catabolized aerobically only in slant with alkaline products
- 23Obligate aerobesO2 necessary for growth and serves as e- acceptor
- 24facultative anaerobescan grow with or without O2 (better with)
capable of fermenting sugars to alcohols or acids - 25microaerophilesneed some O2, but can't survive at atm levels
- 26aerotolerantdon't use O2 for growth, but unaffected by presence
- 27obligate anaerobescannot grow in the presence of O2
- 28acidophilesGROWS at pH <5.5
fermenting bacteria that produce acid byproducts during CHO metabolism - 29neutrophilesGROWS at pH 5.5-8.5
most bacteria, including pathogens - 30alkalophilesGROWS at pH >8.5
protein degraders, typically - 31Psychrophile0-20ºC
(refrigerator spoilage) - 32Mesophile15-45ºC
- 33thermophile40-65ºC
(compost heaps) - 34Hyperthermophile65-120ºC
(hot springs, thermal vents) - 35halophilesgrow at >3% NaCl
- 36extreme halophilesgrow at >15% salt, will not grow at lower concentrations
- 37osmotolerantcan grow across a wide range of salinities
- 38saccharophilesthrive in high sugar concentrations (>50%)
often yeasts and molds - 39virulent phagephage that does NOT undergo a lysogenic life cycle
- 40temperate phagephage that go through lytic and lysogenic life cycle
- 41prophagephage that has its genome integrated into a bacterial genome
- 42cryptic prophageprophage that has lost the ability to undergo lytic cycle
- 43lysogenbacterium with a prophage in its genome
- 44plaque assay determines..phage titre (PFU/mL)
- 45XLD results: Yellowferments lactose or sucrose (hiding decarboxylation)
OR
ferments xylose with no decarboxylation (rare) - 46XLD Redno xylose fermentation
OR
xylose fermentation with decarboxylation
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- MIBO Flashcards on MIBO 3510L Final practical, created by Diamond Sapphire on 26/04/2019.
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