Flashcards
Forensic DNA Analysis I
Cards in this deck (17)
- 1RFLPRestriction Fragment Length Polymorphism
- 2VNTRvariable number tandem repeat
- 3RFLP compared to PCRnot sensitive, 25ng+ DNA needed, sensitive to degradation (alleles are lg), labour intensive & time-consuming
- 4PM+DQA1Polymarker plus DQA1:
limited discrimination
1-2 ng needed
smaller frags amplified (242-245 bases)
labor intensive - 5AMPFLPsAmplified Fragment Length Polymorphisms
poor discrimination
ranged in size 200-1000bp - 6STRShort Tandem Repeats or microsatellites
- 7STR multiplexessimultaneous analysis
high discrimination
0.5-1 ng DNA
100-300 bp range
automation - 8CODISCombined DNA Index System:
DBA database - FBI program
3 tiers: local, state & national (USA) // 2 tiers: local national (CAD)
as of Jan 2017 - 20 CORE loci
(orig. 13 loci) - 9DNA Transfernot whether DNA is from a person but rather HOW it got to where it was found; DNA can be transferred directly or indirectly
- 10Touch DNADNA from epithelial cells, small amounts of DNA,
Factors: Shredders vs. Non-Shedders, type of surface, length of time, previous handlers, presence of other body fluids - 11Locard's Exchange Principlecontact btw 2 items there will ALWAYS be an exchange of material
- 12Standards for Comparison1. cannot enter vic profile into CODIS
2. use elimination samples to prevent non-suspect from being entered
3. direct comparison btw suspect and unknown when eliminations not possible
4. (CAD) request removal of profiles uploaded in error - 13Primary Transfertransfer of DNA from person to object
- 14Secondary TransferDNA is on 1 item/person and is transferred to a different item/person
(ex. Person A, sweaty, shakes hands w\ person B, B opens door handle - person A’s DNA is found on door handle) - 15Sample Collectioncutting, swabbing, dry swab, wet/dry swab
- 16SWGDAMScientific Working Group on DNA Analysis Method
- 17—
Information
- Description
- terms and topics to get through DNA I
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- Sarah Bax
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