Flashcards
Fixation
Cards in this deck (25)
- 1Zinc Salts What it replaces ** 1 ComboAdditive Coag
Replaces Mercury
precipitates (in alcohol)
Corrosive (chloride better than sulfate)
Zinc Formalin - 2Zinc Formalin 4 Types w/ components and *As is: LT IHC Storage, Fast
Aqueous: ZSulf.Form.DI - precip in 70%
Unbuffered Aq.: ZSulf.DI.Form - 6-8hr. fix
Alcoholic: ZChlor.DI.Isopropyl.Form - antigens, nuclei, lipids - 3Gluteraldehyde 2 alternatives w/PAS? Rate? use?PBGTH + Form GTH
NO - dialdehyde, false +
Slow Penetration
EM - 4Alcohols 2 forms */* 2 CombosNon-Additive Coag
Ethyl - gout/urates | Methyl - Smears
Carnoy + Clarke - 5Carnoy Components, Use, AffectEthyl.Chloroform.Acetic
Glycogen, DNA
Shrinks within 4 hrs. - 6Clarke Components100%.Acetic
- 7Acetone Speed/Temp 2 ActionsNon-Additive Coag
Fast Refrigerated
Fixes + Dehydrates - 8Mercuric Chloride Use Frozen Sections? Pigment Color/Clearing 3 CombosAdditive Coag
Mordant
NO FROZEN SECTIONS
Brown- Iodine + Na Thiosulfate
B-5, Zenker, Helly - 9B-5 Components, UseMercury Chlor.Na Acetate. Form
Nuclear Detail - 10Helly Stable or Unstable UseMercuric Chlor.Form.K Dichrom.Na Sulf.DI
Unstable
RBC Preservation - 11Zenker Stable or Unstable UseMercuric Chlor.Acetic.K Dichrom.Na Sulfate.DI
Stable
Nuclear Detail - 12Chromic Acid Use 2 CombosAdditive Coag
Lipid Preservation
Potassium Dichromate + Orth - 13Potassium Dichromate When is it Coag Soft or Hard Wash Before...With Acid (Otherwise Add NonCoag)
Soft
EtOH - 14Orth Components, UseK Dichromate. Na Sulfate. Form. DI
Chromaffin in Cytoplasm - 15Picric Acid Preserves... Wash With... 3 CombosAdditive Coag
Glycogen
50% Before Processing
Bouins, Gendre, Zamboni PAF - 16Bouins Components, UsePicric Acid.Acetic.Form
Mordant for MT and Nuclear Detail - 17Gendre Components, UsePicric Acid.Acetic.Form.95%
Glycogen Preservation - 18Zamboni PAF Components, UsePFA.Picric aq.Na Hydroxide.DI
General use and EM - 19Formalin PFA Difference? pH? Lipids? Pigment PEL/STELAdd NonCoag
No methanol - EM ok
7.5-8.0
Preserved before Processing
Black Acid Hematin (Low pH)
Remove: alc. PA and alkaline alc.
0.75ppm/2.0ppm - 20Osmium Tetroxide Sections? Preserves when to use PELAdditive NonCoag
Thin
Lipids
after fixation (post-fix)
0.002ppm - 21Cupric Salts 1 ComboAdditive Coag
Hollande - 22Hollande Components, Use Rinse before...Copper Acetate.Picric.Form.Acetic.DI
Preserve RBCs
Placing in NBF - 23Glyoxal PAS? % SpeedAdd NonCoag
Yes even though dialdehyde
40%
Fast Fix - 24Acetic Acid Use w/ Alc. Speed/Soft or Hard? Action RBCs?NonAdd Coag
DNA/RNA Preservation
Fast/Soft
Swelling
No RBC Preservation - 25Michel Use, pH, StepsTrasportation
7.0-7.2
PBS rinse, Freeze, Michel
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- HT Histology Flashcards on Fixation, created by Amy Anderson on 13/02/2020.
- Created by
- Amy Anderson
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