20841491
Description
Flashcards by Amy Anderson, updated more than 1 year ago
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Created by Amy Anderson
about 4 years ago
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| Question | Answer |
| Zinc Salts What it replaces ** 1 Combo | Additive Coag Replaces Mercury precipitates (in alcohol) Corrosive (chloride better than sulfate) Zinc Formalin |
| Zinc Formalin 4 Types w/ components and * | As is: LT IHC Storage, Fast Aqueous: ZSulf.Form.DI - precip in 70% Unbuffered Aq.: ZSulf.DI.Form - 6-8hr. fix Alcoholic: ZChlor.DI.Isopropyl.Form - antigens, nuclei, lipids |
| Gluteraldehyde 2 alternatives w/PAS? Rate? use? | PBGTH + Form GTH NO - dialdehyde, false + Slow Penetration EM |
| Alcohols 2 forms */* 2 Combos | Non-Additive Coag Ethyl - gout/urates | Methyl - Smears Carnoy + Clarke |
| Carnoy Components, Use, Affect | Ethyl.Chloroform.Acetic Glycogen, DNA Shrinks within 4 hrs. |
| Clarke Components | 100%.Acetic |
| Acetone Speed/Temp 2 Actions | Non-Additive Coag Fast Refrigerated Fixes + Dehydrates |
| Mercuric Chloride Use Frozen Sections? Pigment Color/Clearing 3 Combos | Additive Coag Mordant NO FROZEN SECTIONS Brown- Iodine + Na Thiosulfate B-5, Zenker, Helly |
| B-5 Components, Use | Mercury Chlor.Na Acetate. Form Nuclear Detail |
| Helly Stable or Unstable Use | Mercuric Chlor.Form.K Dichrom.Na Sulf.DI Unstable RBC Preservation |
| Zenker Stable or Unstable Use | Mercuric Chlor.Acetic.K Dichrom.Na Sulfate.DI Stable Nuclear Detail |
| Chromic Acid Use 2 Combos | Additive Coag Lipid Preservation Potassium Dichromate + Orth |
| Potassium Dichromate When is it Coag Soft or Hard Wash Before... | With Acid (Otherwise Add NonCoag) Soft EtOH |
| Orth Components, Use | K Dichromate. Na Sulfate. Form. DI Chromaffin in Cytoplasm |
| Picric Acid Preserves... Wash With... 3 Combos | Additive Coag Glycogen 50% Before Processing Bouins, Gendre, Zamboni PAF |
| Bouins Components, Use | Picric Acid.Acetic.Form Mordant for MT and Nuclear Detail |
| Gendre Components, Use | Picric Acid.Acetic.Form.95% Glycogen Preservation |
| Zamboni PAF Components, Use | PFA.Picric aq.Na Hydroxide.DI General use and EM |
| Formalin PFA Difference? pH? Lipids? Pigment PEL/STEL | Add NonCoag No methanol - EM ok 7.5-8.0 Preserved before Processing Black Acid Hematin (Low pH) Remove: alc. PA and alkaline alc. 0.75ppm/2.0ppm |
| Osmium Tetroxide Sections? Preserves when to use PEL | Additive NonCoag Thin Lipids after fixation (post-fix) 0.002ppm |
| Cupric Salts 1 Combo | Additive Coag Hollande |
| Hollande Components, Use Rinse before... | Copper Acetate.Picric.Form.Acetic.DI Preserve RBCs Placing in NBF |
| Glyoxal PAS? % Speed | Add NonCoag Yes even though dialdehyde 40% Fast Fix |
| Acetic Acid Use w/ Alc. Speed/Soft or Hard? Action RBCs? | NonAdd Coag DNA/RNA Preservation Fast/Soft Swelling No RBC Preservation |
| Michel Use, pH, Steps | Trasportation 7.0-7.2 PBS rinse, Freeze, Michel |
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