Flashcards
Carbs/Amyloids
Cards in this deck (12)
- 1Periodic Acid Schiff Stains... Action Control Fixative ResultsPolysaccharides/Mucosubstances
oxidizes aldehydes
Kidney Control
NBF/Bouins - NO GTH
+quinoid structure: rose - 2PAS Digestion (PASD) Stains... Action Control Fixative ResultsGlycogen
depolymerize glycogen/glucose
Liver w/ and w/o or Cervix Control
NBF/Alc. Formalin/100%
Glycogen: Bright Rose - 3Mucicarmine Stains... Action Control Fixative ResultsEpithelial Mucin
Al Carmine (+) Attaches to acids in Mucin
GI control
NBF
Mucin: Rose Nuclei: Black
Other: Blue/yellow - 4PAS Hematoxylin (PASH) Fixatives ResultsGlycogen/Mucins
NBF/Zenker
Mucins: Blue Polysacch.: Magenta - 5Alcian Blue w/ Hyalauranidase Stains... Action Control Fixative ResultsEpithelial v CT Mucins
Digests HA and Chondroitin
GI/Umbilical Control
NBF
Mucins: Dark Blue Other: No Stain - 6Alcian Blue pH 1.0 Stains... Action Control Fixative ResultsSulfated Mucosubstances
only sulfated stained due to pH
GI control
NBF/Bouins
Sulf MCSs: pale blue Nuclei: Red
Other: Pink/Red - 7Alcian Blue pH 2.5 Stains... Action Control Fixative ResultsAcid Mucopolysaccharides
Salt linkage
GI Control
NBF/Bouins
Sulf MCSs: Blue Nuclei: Red
Other: Pink/Red - 8Alcian Blue/PAS Stains... Action Control Fixative ResultsNeutral v. Acid MCSs
Acid Alcian - Neutral PAS
Kidney/Cervix Control
NBF/Zenker
Acid: Blue Neutral: Magenta - 9Müller Mowrey Colloidal Iron Stains... Action Control Fixative ResultsCarbox./Sulf. MPSs + Glycogen
Fe+ Absorbed by MPSs Prussian Blue for Iron
GI Control
Acid MPSs: Blue Nuclei: Pink/Red
Cytoplasm: Pink - 10Congo Red Stains... Action/Section Thickness Control Fixative ResultsAmyloids
Binds to Cellulose/8-10um
Alcohol, NBF, Bouins, Zenker, Carnoy
Anything w/ Amyloid Control
Amyloid: pink/red Elastin: pink
Nuclei:Blue - 11Crystal Violet Stains... Action/Section Thickness Control Fixative ResultsAmyloids
Metachromatic/10-12um
Any Amyloid
NBF, Alcohol
Amyloid: purple Other: blue - 12Thioflavin T Stains... Action/Section Thickness Control Fixative ResultsAmyloid
Fluorescent Amyloid
Any Amyloid
NBF
Amyloid Apple Green
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- HT Histology Flashcards on Carbs/Amyloids, created by Amy Anderson on 14/02/2020.
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- Amy Anderson
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