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Sydney Hanula Histology HTL English 43 cards

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Cards in this deck (43)

  1. 1—Alcian Blue, pH 1.0Alcian Blue, pH 1.0
  2. 2Alcian Blue, pH 1.0 Reagents used (4)0.1N HCl acid rinse
    1% Alcian blue w/ HCl acid
    0.1N HCL acid rinse
    Nuclear fast red (NFR)
  3. 3Alcian Blue, pH 1.0 Purposedemonstration of sulfated mucocubstances
  4. 4Alcian Blue, pH 1.0 Fixation (2)10% NBF
    Bouin's solution
  5. 5Alcian Blue, pH 1.0 control tissuecolon, small intestine, appendix
  6. 6Alcian Blue, pH 1.0 resultsSulfated GaGs and sulfomucins - pale blue
    Background - pink to red
  7. 7Alcian Blue, pH 1.0 troubleshooting: 1. Excessive background 2. Blue nuclear staining 3. Weak staining 4. Cloudiness1. Rinse with acetic acid wasn’t sufficient; pH wasn’t maintained;
    2. Blue solution step too long
    3. Dye solution is weak or old; Poor hydration step
    4. Insufficient washing after NFR
  8. 8—Alcian Blue, pH 2.5 w/ HyaluronidaseAlcian Blue, pH 2.5 w/ Hyaluronidase
  9. 9Alcian Blue, pH 2.5 w/ Hyaluronidase reagents used (4)Hyaluronidase
    3% acetic acid
    1% Alcian blue w/ acetic acid
    3% acetic acid
    Nuclear fast red (NFR)
  10. 10Alcian Blue, pH 2.5 w/ Hyaluronidase PurposeDifferentiates between epithelial cells (acidic glycoproteins) and connective tissue (GaGs); carcinoma vs mesothelioma
  11. 11Alcian Blue, pH 2.5 w/ Hyaluronidase PrincipleHyaluronidase digestion results in cleavage of glycosidic linkages and chondroitin; Absence of dramatically reduced staining indicates
  12. 12Alcian Blue, pH 2.5 w/ Hyaluronidase PrincipleHyaluronidase digestion results in cleavage of glycosidic linkages and chondroitin; Absence of dramatically reduced staining indicates
  13. 13Alcian Blue, pH 2.5 w/ Hyaluronidase Fixation (2)10% NBF
    Bouin's solution
  14. 14Alcian Blue, pH 2.5 w/ Hyaluronidase Control tissue2 slides of umbilical cord, intestine or appendix can be used as a 2nd control to demonstrate acidic glycoproteins
  15. 15Alcian Blue, pH 2.5 w/ Hyaluronidase resultsBackground — pink
    Without digestion — deep blue
    With digestion (GaGs and chondroitin will be absent) — loss of staining
  16. 16Alcian Blue, pH 2.5 w/ Hyaluronidase Troubleshooting: 1. excessive background 2. blue nuclear staining 3. weak staining 4. cloudiness1. rinse with acetic acid wasn’t sufficient; pH wasn’t maintained
    2. Blue solution kept too long
    3. Dye solution is weak or old; Poor hydration step
    4. insufficient washing after nuclear fast red
  17. 17—Alcian Blue, pH 2.5Alcian Blue, pH 2.5
  18. 18Alcian Blue, pH 2.5 Reagents used (3)3% acetic acid rinse
    1% Alcian blue w/ 3% acetic acid
    3% acetic acid rinse
    Nuclear fast red (NFR)
  19. 19Alcian Blue, pH 2.5 Purposedemonstration of Gags and acidic glycoproteins (sialomucins and sulfomucins); differentiated by different shades of blue; more specific than colloidal iron
  20. 20Alcian Blue, pH 2.5 PrinciplepH 2.5 is important because sulfated and carboxylated mucopolysaccharides and glycoproteins will all stain
  21. 21Alcian Blue, pH 2.5 Fixation (2)10% NBF
    Bouin's solution
  22. 22Alcian Blue, pH 2.5 Control tissueColon, small intestine, appendix
  23. 23Alcian Blue, pH 2.5 resultsAcidic glycoproteins — blue (more intense blue)
    GaGs — light blue
    Background & nuclei — pink to red
  24. 24Alcian Blue, pH 2.5 Troubleshooting: 1. Excessive background 2. Blue nuclear staining 3. Weak staining 4. Cloudiness1. rinse with acetic acid wasn’t sufficient; pH wasn’t maintained
    2. blue solution step too long
    3. dye solution is weak or old; poor hydration step
    4. insufficient washing after NFR
  25. 25—Alcian Blue -- PASAlcian Blue -- PAS
  26. 26Alcian Blue -- PAS reagents used (5)3% acetic acid
    1% alcian blue w/ acetic acid
    0.5% periodic acid
    schiff reagent
    hematoxylin
  27. 27Alcian Blue - PAS purposeDifferentiate neutral and acidic glycans; Acidic mucosubstances are stained with Alcian blue; Neutral substances are stained with PAS
  28. 28Alcian Blue - PAS fixation (2)10% NBF
    Bouin's
  29. 29Alcian Blue - PAS control tissuekidney or cervix
  30. 30Alcian Blue - PAS results- GaGs & acidic glycoproteins: blue
    - Neutral mucosubstances & glycogen: magenta
    - Nuclei: purple
  31. 31—Alcian Yellow — toluidine blueAlcian Yellow — toluidine blue
  32. 32Alcian Yellow — toluidine blue reagents used (4)- periodic acid
    - potassium/sodium metabisulfite
    - alcian yellow
    - T. blue
  33. 33Alcian Yellow — toluidine blue purposestains H. Pylori
  34. 34Alcian Yellow — toluidine blue fixation10% NBF
  35. 35Alcian Yellow — toluidine blue control tissueKnown H. Pylori positive tissue
  36. 36Alcian Yellow — toluidine blue results- H. Pylori: blue
    - Mucin: yellow
    - Background: pale blue
  37. 37—Aldehyde Fuchsin Elastic StainAldehyde Fuchsin Elastic Stain
  38. 38Aldehyde Fuchsin Elastic Stain reagents used (3)- aldehyde fuchsin solution
    - 70% alcohol
    - light green or van gieson
  39. 39Aldehyde Fuchsin Elastic Stain purposeto demonstrate elastic fibers
  40. 40Aldehyde Fuchsin Elastic Stain fixation10% NBF
    acid chromates
  41. 41Aldehyde Fuchsin Elastic Stain control tissueaorta, skin, muscular artery
  42. 42Aldehyde Fuchsin Elastic Stain results- elastic fibers: deep blue to purple
    - background: green
  43. 43Aldehyde Fuchsin Elastic Stain troubleshooting: 1: inadequate elastic staining 2: poor contrast1: old/overused solution
    2: overpowering counterstain

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HTL Histology Flashcards on Untitled, created by Sydney Hanula on 15/10/2023.
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Sydney Hanula
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